Wnt5b-Ryk pathway provides directional signals to regulate gastrulation movement

Shengda Lin; Lisa M Baye; Trudi A Westfall; Diane C Slusarski

doi:10.1083/jcb.200912128

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Wnt5b-Ryk pathway provides directional signals to regulate gastrulation movement

Journal article

Open access

Peer reviewed

Wnt5b-Ryk pathway provides directional signals to regulate gastrulation movement

Shengda Lin, Lisa M Baye, Trudi A Westfall and Diane C Slusarski

The Journal of cell biology, Vol.190(2), pp.263-278

07/26/2010

DOI: 10.1083/jcb.200912128

PMCID: PMC2930277

PMID: 20660632

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Abstract

Noncanonical Wnts are largely believed to act as permissive cues for vertebrate cell movement via Frizzled (Fz). In addition to Fz, Wnt ligands are known to regulate neurite outgrowth through an alternative receptor related to tyrosine kinase (Ryk). However, Wnt-Ryk signaling during embryogenesis is less well characterized. In this study, we report a role for Wnt5b as an instructive cue to regulate gastrulation movements through Ryk. In zebrafish, Ryk deficiency impairs Wnt5b-induced Ca(2+) activity and directional cell movement. Wnt5b-Ryk signaling promotes polarized cell protrusions. Upon Wnt5b stimulation, Fz2 but not Ryk recruits Dishevelled to the cell membrane, suggesting that Fz2 and Ryk mediate separate pathways. Using co-culture assays to generate directional Wnt5b cues, we demonstrate that Ryk-expressing cells migrate away from the Wnt5b source. We conclude that full-length Ryk conveys Wnt5b signals in a directional manner during gastrulation.

Phenotype

Embryo, Nonmammalian - anatomy & histology

Receptors, G-Protein-Coupled - metabolism

Calcium - metabolism

Coculture Techniques

Humans

Gene Expression Profiling

Wnt-5a Protein

Wnt Proteins - metabolism

Zebrafish - embryology

Recombinant Fusion Proteins - metabolism

Cell Movement - physiology

Wnt Proteins - genetics

Embryo, Nonmammalian - physiology

Gastrulation

Zebrafish Proteins - metabolism

Cells, Cultured

Frizzled Receptors - metabolism

Receptor Protein-Tyrosine Kinases - metabolism

Animals

Frizzled Receptors - genetics

Receptor Protein-Tyrosine Kinases - genetics

Zebrafish - metabolism

Recombinant Fusion Proteins - genetics

Signal Transduction - physiology

Receptors, G-Protein-Coupled - genetics

Zebrafish Proteins - genetics

Details

Title: Subtitle: Wnt5b-Ryk pathway provides directional signals to regulate gastrulation movement
Creators: Shengda Lin - University of Iowa
Lisa M Baye
Trudi A Westfall - University of Iowa, Biology
Diane C Slusarski - University of Iowa, Iowa Neuroscience Institute
Resource Type: Journal article
Publication Details: The Journal of cell biology, Vol.190(2), pp.263-278
DOI: 10.1083/jcb.200912128
PMID: 20660632
PMCID: PMC2930277
ISSN: 0021-9525
eISSN: 1540-8140
Grant note: T32 HL007121 / NHLBI NIH HHS CA112369 / NCI NIH HHS R01 CA112369 / NCI NIH HHS
Language: English
Date published: 07/26/2010
Academic Unit: Iowa Neuroscience Institute; Biology
Record Identifier: 9984071768502771

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