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Xanthosoma sagittifolium Tubers Contain a Lectin with Two Different Types of Carbohydrate-binding Sites
Journal article   Open access   Peer reviewed

Xanthosoma sagittifolium Tubers Contain a Lectin with Two Different Types of Carbohydrate-binding Sites

Hanqing Mo, Kevin G Rice, David L Evers, Harry C Winter, Willy J Peumans, Els J.M Van Damme and Irwin J Goldstein
The Journal of biological chemistry, Vol.274(47), pp.33300-33305
11/1999
DOI: 10.1074/jbc.274.47.33300
PMID: 10559206
url
https://doi.org/10.1074/jbc.274.47.33300View
Published (Version of record) Open Access

Abstract

An unusual lectin possessing two distinctly different types of carbohydrate-combining sites was purified from tubers of Xanthosoma sagittifolium L. by consecutive passage through two affinity columns, i.e. asialofetuin-Sepharose and invertase-Sepharose. SDS-polyacrylamide gel electrophoresis, N-terminal amino acid sequencing, and gel filtration chromatography of the purified lectin showed that the X. sagittifolium lectin is a heterotetrameric protein composed of four 12-kDa subunits (alpha(2)beta(2)) linked by noncovalent bonds. The results obtained by quantitative precipitation and hapten inhibition assays revealed that the lectin has two different types of carbohydrate-combining sites: one type for oligomannoses, which preferentially binds to a cluster of nonreducing terminal alpha1,3-linked mannosyl residues, and the other type for complex N-linked carbohydrates, which best accommodates a non-sialylated, triantennary oligosaccharide with N-acetyllactosamine (i.e. Galbeta1,4GlcNAc-) or lacto-N-biose (i.e. Galbeta1,3GlcNAc-) groups at its three nonreducing termini.

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