Yeast bZip proteins mediate pleiotropic drug and metal resistance

Ailing Wu; John A Wemmie; Nicholas P Edgington; Mark Goebl; Jose L Guevara; W Scott Moye-Rowley

doi:10.1016/S0021-9258(17)46705-6

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Yeast bZip proteins mediate pleiotropic drug and metal resistance

Journal article

Open access

Peer reviewed

Yeast bZip proteins mediate pleiotropic drug and metal resistance

Ailing Wu, John A Wemmie, Nicholas P Edgington, Mark Goebl, Jose L Guevara and W Scott Moye-Rowley

The Journal of biological chemistry, Vol.268(25), pp.18850-18858

09/05/1993

DOI: 10.1016/S0021-9258(17)46705-6

PMID: 8360174

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Abstract

Saccharomyces cerevisiae contains a group of transcription factors related to mammalian c-Jun. This yeast Jun-family of proteins consists of GCN4, a regulator of genes involved in amino acid biosynthesis, and yAP-1, a factor conferring pleiotropic drug resistance when overexpressed. In the work described here, we show that a third member of the yeast Jun-family exists. This protein has been designated CAD1 and provides resistance to cadmium when present on a high-copy plasmid. CAD1 and yAP-1 are related in their amino-terminal DNA binding domains and can recognize the same DNA target site in vitro. Overproduction of CAD1 leads to transcriptional activation of an artificial reporter gene in delta yap1 cells. High level production of either CAD1 or yAP-1 causes cells to acquire a pleiotropic drug-resistant phenotype and to be able to tolerate normally toxic levels of iron chelators and zinc. Surprisingly, disruption of the CAD1 gene has no effect on the normal cellular resistance to cadmium but delta yap1 mutants are hypersensitive to this cytotoxic metal. The cadmium hypersensitivity of the delta yap1 mutant described here indicates that one major role of YAP1 in the yeast cell is to mediate resistance to this metal.

Gene Expression

Mutation

Plasmids

Fungal Proteins - chemistry

Saccharomyces cerevisiae - genetics

Transcriptional Activation

Molecular Sequence Data

Saccharomyces cerevisiae - metabolism

Base Sequence

Genes, Fungal

Binding Sites

Recombinant Proteins - metabolism

Amino Acid Sequence

DNA-Binding Proteins - physiology

Transcription Factors - physiology

Cadmium - pharmacology

Fungal Proteins - genetics

Transcription Factors - genetics

Cycloheximide - pharmacology

Saccharomyces cerevisiae - chemistry

Sequence Homology, Amino Acid

Transcription Factors - metabolism

Drug Resistance, Microbial

DNA, Fungal - metabolism

Saccharomyces cerevisiae Proteins

Plant Proteins

Zinc - pharmacology

Basic-Leucine Zipper Transcription Factors

Fungal Proteins - metabolism

G-Box Binding Factors

Details

Title: Subtitle: Yeast bZip proteins mediate pleiotropic drug and metal resistance
Creators: Ailing Wu - Program in Molecular Biology, University of Iowa, Iowa City 52242
John A Wemmie
Nicholas P Edgington
Mark Goebl
Jose L Guevara
W Scott Moye-Rowley
Resource Type: Journal article
Publication Details: The Journal of biological chemistry, Vol.268(25), pp.18850-18858
DOI: 10.1016/S0021-9258(17)46705-6
PMID: 8360174
NLM abbreviation: J Biol Chem
ISSN: 0021-9258
eISSN: 1083-351X
Publisher: United States
Language: English
Date published: 09/05/1993
Academic Unit: Molecular Physiology and Biophysics; Psychiatry; Iowa Neuroscience Institute; Neurosurgery; Internal Medicine
Record Identifier: 9984003923202771

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