Journal article
Young thrombocytes initiate the formation of arterial thrombi in zebrafish
Blood, Vol.106(1), pp.118-124
07/01/2005
DOI: 10.1182/blood-2004-10-4118
PMCID: PMC1895120
PMID: 15769888
Abstract
The zebrafish system is an excellent vertebrate genetic model to study hemostasis and thrombosis because saturation mutagenesis screens can identify novel genes that play a role in this vital physiologic pathway. To study hemostatic mutations, it is important to understand the physiology of zebrafish hemostasis and thrombosis. Previously, we identified zebrafish thrombocytes and have shown that they participate in arterial thrombus formation. Here, we recognized 2 populations of thrombocytes distinguishable by DiI-C18 (DiI) staining. DiI
+
thrombocytes have a high density of adhesive receptors and are functionally more active than DiI
–
thrombocytes. We classified DiI
+
thrombocytes as young and DiI
–
thrombocytes as mature thrombocytes. We found young and mature thrombocytes each formed independent clusters and that young thrombocytes clustered first. We have also shown that young thrombocytes initiate arterial thrombus formation. We propose that due to the increased adhesive receptor density on young thrombocytes, they adhere first to the subendothelial matrix, get activated rapidly, release agonists, and recruit more young thrombocytes, which further release more agonists. This increase in agonists activates the less active mature thrombocytes, drawing them to the growing thrombus. Since arterial thrombus formation is a fundamental hemostatic event, this mechanism may be conserved in mammals and may open new avenues for prevention of arterial thrombosis.
Details
- Title: Subtitle
- Young thrombocytes initiate the formation of arterial thrombi in zebrafish
- Creators
- Bijoy Thattaliyath - From the Department of Cellular and Structural Biology, The University of Texas Health Science Center, San Antonio TXMatthew Cykowski - From the Department of Cellular and Structural Biology, The University of Texas Health Science Center, San Antonio TXPudur Jagadeeswaran - From the Department of Cellular and Structural Biology, The University of Texas Health Science Center, San Antonio TX
- Resource Type
- Journal article
- Publication Details
- Blood, Vol.106(1), pp.118-124
- DOI
- 10.1182/blood-2004-10-4118
- PMID
- 15769888
- PMCID
- PMC1895120
- NLM abbreviation
- Blood
- ISSN
- 0006-4971
- eISSN
- 1528-0020
- Publisher
- The American Society of Hematology
- Language
- English
- Date published
- 07/01/2005
- Academic Unit
- Cardiology; Stead Family Department of Pediatrics
- Record Identifier
- 9984093217102771
Metrics
14 Record Views