Journal article
ZAP-70 Is Essential for the T Cell Antigen Receptor-induced Plasma Membrane Targeting of SOS and Vav in T Cells
The Journal of biological chemistry, Vol.275(8), pp.5966-5975
02/25/2000
DOI: 10.1074/jbc.275.8.5966
PMID: 10681590
Abstract
Translocation of the SOS and Vav GDP/GTP exchange factors proximal to Ras and Rac GTPases localized in the plasma membrane glycolipid-enriched microdomains is a pivotal step required for T cell antigen receptor-induced T cell activation. Here we demonstrate that the T cell antigen receptor zeta-chain-associated ZAP-70 kinase and T cell antigen receptor zeta-chain immunoreceptor tyrosine-based activation motifs are essential for the membrane recruitment of SOS and Vav. Plasma membrane targeting of SOS or Vav begins with the assembly of ZAP-70 with Grb-2 and SOS. The subsequent tyrosine phosphorylation of LAT (linker for activation of T cell) by ZAP-70 leads to a shift in equilibrium from the ZAP-70.Grb-2.SOS(Vav) complex to the (Vav)SOS.Grb-2.LAT complex. This shift results in the targeting of SOS and Vav into glycolipid-enriched microdomains and initiation of the Ras and Rac signaling cascades involved in T cell activation, proliferation, and cytokine production.
Details
- Title: Subtitle
- ZAP-70 Is Essential for the T Cell Antigen Receptor-induced Plasma Membrane Targeting of SOS and Vav in T Cells
- Creators
- Konstantin V SalojinJian ZhangCraig MeagherTerry L Delovitch
- Resource Type
- Journal article
- Publication Details
- The Journal of biological chemistry, Vol.275(8), pp.5966-5975
- DOI
- 10.1074/jbc.275.8.5966
- PMID
- 10681590
- NLM abbreviation
- J Biol Chem
- ISSN
- 0021-9258
- eISSN
- 1083-351X
- Language
- English
- Date published
- 02/25/2000
- Academic Unit
- Pathology
- Record Identifier
- 9984047879802771
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