ZO-1 recruitment to α-catenin--a novel mechanism for coupling the assembly of tight junctions to adherens junctions

Jessica L Maiers; Xiao Peng; Alan S Fanning; Kris A DeMali

doi:10.1242/jcs.126565

Back

ZO-1 recruitment to α-catenin--a novel mechanism for coupling the assembly of tight junctions to adherens junctions

Journal article

Open access

Peer reviewed

ZO-1 recruitment to α-catenin--a novel mechanism for coupling the assembly of tight junctions to adherens junctions

Jessica L Maiers, Xiao Peng, Alan S Fanning and Kris A DeMali

Journal of cell science, Vol.126(Pt 17), pp.3904-3915

09/01/2013

DOI: 10.1242/jcs.126565

PMCID: PMC3757330

PMID: 23813953

Files and links (1)

url

https://doi.org/10.1242/jcs.126565View

Published (Version of record) Open Access

Abstract

The formation of a barrier between epithelial cells is a fundamental determinant of cellular homeostasis, protecting underlying cells against pathogens, dehydration and damage. Assembly of the tight junction barrier is dependent upon neighboring epithelial cells binding to one another and forming adherens junctions, but the mechanism for how these processes are linked is poorly understood. Using a knockdown and substitution system, we studied whether ZO-1 binding to α-catenin is required for coupling tight junction assembly to the formation of adherens junctions. We found that preventing ZO-1 binding to α-catenin did not appear to affect adherens junctions. Rather the assembly and maintenance of the epithelial barrier were disrupted. This disruption was accompanied by alterations in the mobility of ZO-1 and the organization of the actin cytoskeleton. Thus, our study identifies α-catenin binding to ZO-1 as a new mechanism for coupling the assembly of the epithelial barrier to cell-to-cell adhesion.

Tight Junctions - metabolism

Cell Line

Microscopy, Electron, Transmission

Cell Adhesion - genetics

Protein Binding - genetics

Actin Cytoskeleton - metabolism

Epithelial Cells - metabolism

Fluorescence Recovery After Photobleaching

alpha Catenin - metabolism

Electric Impedance

Mutation - genetics

Adherens Junctions - metabolism

Animals

Cell Adhesion - physiology

Dogs

Madin Darby Canine Kidney Cells

Zonula Occludens-1 Protein - metabolism

Details

Title: Subtitle: ZO-1 recruitment to α-catenin--a novel mechanism for coupling the assembly of tight junctions to adherens junctions
Creators: Jessica L Maiers - The Interdisciplinary Graduate Program in Molecular and Cellular Biology, University of Iowa Roy J. and Lucille A. Carver College of Medicine, Iowa City, IA 52242, USA
Xiao Peng
Alan S Fanning
Kris A DeMali
Resource Type: Journal article
Publication Details: Journal of cell science, Vol.126(Pt 17), pp.3904-3915
DOI: 10.1242/jcs.126565
PMID: 23813953
PMCID: PMC3757330
NLM abbreviation: J Cell Sci
ISSN: 0021-9533
eISSN: 1477-9137
Publisher: England
Grant note: DK061397 / NIDDK NIH HHS R56 DK061397 / NIDDK NIH HHS R01 DK061397 / NIDDK NIH HHS
Language: English
Date published: 09/01/2013
Academic Unit: Dermatology; Fraternal Order of Eagles Diabetes Research Center; Biochemistry and Molecular Biology
Record Identifier: 9984025283502771

Metrics

30 Record Views

61 Times Cited - Web of Science