Zebrafish Nkd1 promotes Dvl degradation and is required for left-right patterning

Igor Schneider; Patricia N Schneider; Sarah W Derry; Shengda Lin; Lacy J Barton; Trudi Westfall; Diane C Slusarski

doi:10.1016/j.ydbio.2010.08.040

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Zebrafish Nkd1 promotes Dvl degradation and is required for left-right patterning

Journal article

Open access

Peer reviewed

Zebrafish Nkd1 promotes Dvl degradation and is required for left-right patterning

Igor Schneider, Patricia N Schneider, Sarah W Derry, Shengda Lin, Lacy J Barton, Trudi Westfall and Diane C Slusarski

Developmental biology, Vol.348(1), pp.22-33

12/01/2010

DOI: 10.1016/j.ydbio.2010.08.040

PMCID: PMC2976797

PMID: 20858476

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Abstract

The establishment of the left-right (LR) axis in zebrafish embryos relies on signals from the dorsal forerunner cells (DFC) and the Kupffer's vesicle (KV). While the Wnt signaling network influences many aspects of embryonic development, its precise role in LR patterning is still unclear. One branch of the Wnt network leads to stabilization of β-catenin and activation of downstream target genes. Other Wnt ligands appear to act independently of β-catenin to modulate calcium release and influence cell polarity. Central to regulation of β-catenin and coordination of convergent extension (CE) movements is Dishevelled (Dvl). Naked Cuticle (Nkd) binds Dvl and modulates β-catenin-dependent and independent Wnt signaling. Here, we analyze the expression patterns of three zebrafish Nkd homologs and find enriched expression of nkd1 in DFCs and KV. Dvl is degraded upon Nkd1 overexpression in zebrafish. Knockdown of Nkd1 specifically in the DFC results in β-catenin nuclear localization and transcriptional activation as well as alterations to DFC migration, KV formation, ciliogenesis and LR patterning. Furthermore, we identify asymmetric expression of the Nodal antagonist charon around the KV and show that Nkd1 knockdown impacts asymmetric charon expression. Our findings show that Nkd1 acts as a β-catenin antagonist in the DFCs necessary for LR patterning.

Molecular Sequence Data

Phosphoproteins - metabolism

Zebrafish - embryology

Gene Knockdown Techniques

Gene Expression Regulation, Developmental

Cilia - ultrastructure

beta Catenin - physiology

Protein Stability

Dishevelled Proteins

Carrier Proteins - physiology

Zebrafish Proteins - biosynthesis

Oligonucleotides, Antisense - pharmacology

Animals, Genetically Modified

Zebrafish - genetics

Zebrafish Proteins - physiology

Body Patterning - physiology

Carrier Proteins - genetics

Cell Movement - drug effects

Zebrafish Proteins - deficiency

Animals

Zebrafish Proteins - isolation & purification

Zebrafish - physiology

Zebrafish Proteins - genetics

Adaptor Proteins, Signal Transducing - metabolism

Body Patterning - genetics

Details

Title: Subtitle: Zebrafish Nkd1 promotes Dvl degradation and is required for left-right patterning
Creators: Igor Schneider - Department of Biology, University of Iowa, Iowa City, IA 52242, USA
Patricia N Schneider
Sarah W Derry
Shengda Lin
Lacy J Barton
Trudi Westfall
Diane C Slusarski
Resource Type: Journal article
Publication Details: Developmental biology, Vol.348(1), pp.22-33
DOI: 10.1016/j.ydbio.2010.08.040
PMID: 20858476
PMCID: PMC2976797
NLM abbreviation: Dev Biol
ISSN: 0012-1606
eISSN: 1095-564X
Publisher: United States
Grant note: CA112369 / NCI NIH HHS R01 CA112369 / NCI NIH HHS R01 CA112369-03 / NCI NIH HHS
Language: English
Date published: 12/01/2010
Academic Unit: Iowa Neuroscience Institute; Biology; Fraternal Order of Eagles Diabetes Research Center
Record Identifier: 9984070218302771

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