Journal article
Zebrafish and Frog Models of Mycobacterium marinum Infection
Current protocols in microbiology (Online), Vol.3(1), pp.10B.2.1-10B.2.33
12/2006
DOI: 10.1002/0471729256.mc10b02s3
PMID: 18770575
Abstract
Mycobacterium marinum infection of poikilothermic animals, such as fish and frogs, results in chronic granulomatous diseases that bear many similarities to mycobacterioses in mammals, including tuberculosis. This unit describes three animal models of M. marinum infection that can be used to study basic aspects of Mycobacterium‐host interactions and granuloma development, as well as trafficking of immune cells in host tissues. Protocols are included that describe intraperitoneal infection of adult leopard frogs (Rana pipiens) and zebrafish (Danio rerio). Protocols also describe subsequent monitoring of the infection by enumeration of bacterial cfu, mean time to death, or visual examination of infected tissue using both conventional histological stains and fluorescence microscopy of fluorescently marked bacteria. Furthermore, protocols are included that describe the infection of embryonic zebrafish and the subsequent analysis of the infection in real time using DIC and fluorescence microscopy.
Details
- Title: Subtitle
- Zebrafish and Frog Models of Mycobacterium marinum Infection
- Creators
- Christine L Cosma - University of Washington School of MedicineLaura E Swaim - University of Washington School of MedicineHannah Volkman - University of Washington School of MedicineLalita Ramakrishnan - University of Washington School of MedicineJ. Muse Davis - Emory University
- Resource Type
- Journal article
- Publication Details
- Current protocols in microbiology (Online), Vol.3(1), pp.10B.2.1-10B.2.33
- DOI
- 10.1002/0471729256.mc10b02s3
- PMID
- 18770575
- ISSN
- 1934-8525
- eISSN
- 1934-8533
- Number of pages
- 33
- Language
- English
- Date published
- 12/2006
- Academic Unit
- Stead Family Department of Pediatrics; Infectious Disease (Pediatrics)
- Record Identifier
- 9984093215002771
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