Zebrafish prickle, a modulator of noncanonical Wnt/Fz signaling, regulates gastrulation movements

Michael T Veeman; Diane C Slusarski; Ajamete Kaykas; Sarah Hallagan Louie; Randall T Moon

doi:10.1016/S0960-9822(03)00240-9

Back

Zebrafish prickle, a modulator of noncanonical Wnt/Fz signaling, regulates gastrulation movements

Journal article

Open access

Peer reviewed

Zebrafish prickle, a modulator of noncanonical Wnt/Fz signaling, regulates gastrulation movements

Michael T Veeman, Diane C Slusarski, Ajamete Kaykas, Sarah Hallagan Louie and Randall T Moon

Current biology, Vol.13(8), pp.680-685

04/15/2003

DOI: 10.1016/S0960-9822(03)00240-9

PMID: 12699626

Files and links (1)

url

https://doi.org/10.1016/S0960-9822(03)00240-9View

Published (Version of record) Open Access

Abstract

In addition to the canonical Wnt/beta-catenin signaling pathway, at least two noncanonical Wnt/Fz pathways have been described: the planar cell polarity (PCP) pathway in Drosophila [1] and the Wnt/calcium pathway in vertebrate embryos [2]. Recent work suggests that a vertebrate pathway homologous to the PCP pathway acts to regulate the convergent extension movements of gastrulation [3-7]. To further test this hypothesis, we have identified two zebrafish homologs of the Drosophila PCP gene prickle (pk) [8], both of which show discrete and dynamic expression patterns during gastrulation. Both gain and loss of pk1 function cause defects in convergent extension. Pk1 localizes to both the cytoplasm and the cell membrane, and its normal localization is partially dependent on its C-terminal prenylation motif. At the cell membrane, Pk1 is frequently localized asymmetrically around the cell and can colocalize with the signaling molecule Dishevelled (Dsh). In overexpression assays, Pk1 is able to activate AP-1-mediated transcription and inhibit activation of Wnt/beta-catenin signaling. Like noncanonical Wnts [9-10], overexpression of Pk1 increases the frequency of calcium transients in zebrafish blastulae. Our results support the idea that a vertebrate PCP pathway regulates gastrulation movements and suggest that there is overlap between the PCP and Wnt/calcium pathways.

Frizzled Receptors

Immunohistochemistry

Gene Expression

LIM Domain Proteins

Glycoproteins - metabolism

Zebrafish Proteins

Wnt Proteins

DNA-Binding Proteins - genetics

Phosphoproteins - metabolism

Zebrafish - embryology

Cell Movement - genetics

Cell Movement - physiology

Gastrula - physiology

Adaptor Proteins, Signal Transducing

Animals

In Situ Hybridization

Proteins - metabolism

Zebrafish - physiology

Gastrula - metabolism

Signal Transduction - physiology

Drosophila Proteins - genetics

Dishevelled Proteins

Details

Title: Subtitle: Zebrafish prickle, a modulator of noncanonical Wnt/Fz signaling, regulates gastrulation movements
Creators: Michael T Veeman - Department of Pharmacology, Center for Developmental Biology, Howard Hughes Medical Institute, University of Washington School of Medicine, Seattle, WA 98195, USA
Diane C Slusarski
Ajamete Kaykas
Sarah Hallagan Louie
Randall T Moon
Resource Type: Journal article
Publication Details: Current biology, Vol.13(8), pp.680-685
Publisher: England
DOI: 10.1016/S0960-9822(03)00240-9
PMID: 12699626
ISSN: 0960-9822
eISSN: 1879-0445
Grant note: R01 HO 27525 / NHLBI NIH HHS
Language: English
Date published: 04/15/2003
Academic Unit: Iowa Neuroscience Institute; Biology
Record Identifier: 9983992038202771

Metrics

25 Record Views

778 Times Cited - Web of Science

See more details