Journal article
Zinc Is Externalized Rather than Released during Synaptic Transmission
ACS chemical neuroscience, Vol.1(11), pp.728-736
09/09/2010
DOI: 10.1021/cn100065s
PMCID: PMC3017368
PMID: 21221416
Abstract
The synaptic vesicles of some glutamatergic terminals contain a high concentration of zinc that serves functions that remain obscure. In this publication, we have used the membrane permeant zinc fluophore, ZnAF-2, to determine whether zinc is released during the course of synaptic transmission. Stimulation of the slices either with high potassium or electrically, leads to an increase in fluorescence that long outlasts the stimulus and remains elevated for many minutes. We demonstrate that this response is inconsistent with the free release of zinc but is consistent with the presentation of zinc coordinated to macromolecules within the exocytosed vesicles to the extracellular space; a process we term “externalization”. Our data suggests a novel mechanism of synaptic transmission at zinc-rich glutamatergic terminals that distinguishes them from their metal free counterparts.
Details
- Title: Subtitle
- Zinc Is Externalized Rather than Released during Synaptic Transmission
- Creators
- Irma Nydegger - Department of BiologySean M Rumschik - Department of BiologyAlan R Kay - Department of Biology
- Resource Type
- Journal article
- Publication Details
- ACS chemical neuroscience, Vol.1(11), pp.728-736
- DOI
- 10.1021/cn100065s
- PMID
- 21221416
- PMCID
- PMC3017368
- NLM abbreviation
- ACS Chem Neurosci
- ISSN
- 1948-7193
- eISSN
- 1948-7193
- Publisher
- American Chemical Society
- Language
- English
- Date published
- 09/09/2010
- Academic Unit
- Iowa Neuroscience Institute; Biology
- Record Identifier
- 9984068345402771
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