Journal article
cAMP stimulation of HCO3- secretion across airway epithelia
Journal of the Pancreas, Vol.2(4 Suppl), pp.291-293
07/2001
PMID: 11875274
Abstract
To test for the presence of HCO(3)(-) transport across airway epithelia, we measured short-circuit current in primary cultures of canine and human airway epithelia bathed in a Cl(-)-free, HCO(3)(-)/CO(2)-buffered solution. cAMP agonists stimulated a secretory current that was likely carried by HCO(3)(-) because it was absent in HCO(3)(-)-free solutions. In addition, the cAMP-stimulated current was inhibited by the carbonic anhydrase inhibitor, acetazolamide, and by the apical addition of a blocker of cystic fibrosis transmembrane conductance regulator (CFTR), diphenylamine-2-carboxylate. The current was dependent on Na(+) because it was inhibited by removing Na(+) from the submucosal solution and by inhibition of the Na(+)-K(+)-ATPase with ouabain. The cAMP-stimulated current was absent in cystic fibrosis (CF) airway epithelia. These data suggest that cAMP agonists can stimulate HCO(3)(-) secretion across airway epithelia and that CFTR may provide a conductive pathway for HCO(3)(-) movement across the apical membrane.
Details
- Title: Subtitle
- cAMP stimulation of HCO3- secretion across airway epithelia
- Creators
- M J Welsh - Howard Hughes Medical Institute and Departments of Internal Medicine and Pediatrics, University of Iowa College of Medicine, Iowa City, IA 52242, USA. mjwelsh@blue.weeg.uiowa.eduJ J Smith
- Resource Type
- Journal article
- Publication Details
- Journal of the Pancreas, Vol.2(4 Suppl), pp.291-293
- PMID
- 11875274
- ISSN
- 1590-8577
- eISSN
- 1590-8577
- Language
- English
- Date published
- 07/2001
- Academic Unit
- Neurology; Molecular Physiology and Biophysics; Pulmonary, Critical Care, and Occupational Medicine; Stead Family Department of Pediatrics; Fraternal Order of Eagles Diabetes Research Center; Neurosurgery; Internal Medicine
- Record Identifier
- 9984259506602771
Metrics
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