Journal article
cDNA expressed sequence tags of Trypanosoma brucei rhodesiense provide new insights into the biology of the parasite
Molecular and biochemical parasitology, Vol.73(1), pp.75-90
1995
DOI: 10.1016/0166-6851(95)00098-L
PMID: 8577350
Abstract
A total of 518 expressed sequence tags (ESTs) have been generated from clones randomly selected from a cDNA library and a spliced leader sub-library of a
Trypanosoma brucei rhodesiense bloodstream clone. 205 (39%) of the clones were identified based on matches to 113 unique genes in the public databases. Of these, 71 cDNAs display significant similarities to genes in unrelated organisms encoding metabolic enzymes, signal transduction proteins, transcription factors, ribosomal proteins, histones, a proliferation-associated protein and thimet oligopeptidase, among others. 313 of the cDNAs are not related to any other sequences in the databases. These cDNA ESTs provide new avenues of research for exploring both the novel trypanosome-specific genes and the genome organization of this parasite, as well as a resource for identifying trypanosome homologs to genes expressed in other organisms.
Details
- Title: Subtitle
- cDNA expressed sequence tags of Trypanosoma brucei rhodesiense provide new insights into the biology of the parasite
- Creators
- Najib M.A El-Sayed - Department of Biochemistry, University of Iowa, Iowa City, IA 52242, USAClara M Alarcon - Human Nutrition Program, University of Iowa, Iowa City, IA 52242, USAJohn C Beck - Department of Pediatrics, University of Iowa, Iowa City, IA 52242, USAVal C Sheffield - Department of Pediatrics, University of Iowa, Iowa City, IA 52242, USAJohn E Donelson - Department of Biochemistry, University of Iowa, Iowa City, IA 52242, USA
- Resource Type
- Journal article
- Publication Details
- Molecular and biochemical parasitology, Vol.73(1), pp.75-90
- Publisher
- Elsevier B.V
- DOI
- 10.1016/0166-6851(95)00098-L
- PMID
- 8577350
- ISSN
- 0166-6851
- eISSN
- 1872-9428
- Language
- English
- Date published
- 1995
- Academic Unit
- Stead Family Department of Pediatrics; Iowa Neuroscience Institute; Medical Genetics and Genomics; Biochemistry and Molecular Biology; Ophthalmology and Visual Sciences
- Record Identifier
- 9984065377502771
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