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hAG-2, the Human Homologue of theXenopus laevisCement Gland Gene XAG-2, Is Coexpressed with Estrogen Receptor in Breast Cancer Cell Lines
Journal article   Open access   Peer reviewed

hAG-2, the Human Homologue of theXenopus laevisCement Gland Gene XAG-2, Is Coexpressed with Estrogen Receptor in Breast Cancer Cell Lines

Devon A. Thompson and Ronald J. Weigel
Biochemical and biophysical research communications, Vol.251(1), pp.111-116
10/09/1998
DOI: 10.1006/bbrc.1998.9440
url
https://doi.org/10.1006/bbrc.1998.9440View
Published (Version of record) Open Access

Abstract

hAG-2, the human homologue of theXenopus laeviscement gland geneXenopusAnterior Gradient-2 (XAG-2), has been found to be coexpressed with estrogen receptor (ER) in breast cancer cell lines by using suppression subtractive hybridization. Sequence analysis of clones obtained from a human breast adenocarcinoma MCF7 cDNA library suggests that at least three mRNA transcripts, differing in their 3′-untranslated regions, are transcribed from the hAG-2 gene. Northern blot analyses identified two principal hAG-2 signals in all ER-positive breast carcinoma cell lines that were absent in cell lines not expressing, or minimally expressing, ER. Coexpression of hAG-2 with ER suggests that hAG-2 may be involved in the tumor biology specific to the well-differentiated phenotype of hormonally-responsive breast cancers.
breast cancer differentiation estrogen receptor Northern analysis

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