Journal article
iNSC suppress macrophage-induced inflammation by repressing COX-2
In vitro cellular & developmental biology. Animal, Vol.51(2), pp.157-164
02/01/2015
DOI: 10.1007/s11626-014-9816-4
PMID: 25248435
Abstract
Brain inflammation causes cell damage and death in diseases such as Alzheimer's and Parkinson's. In this study, we investigated whether early induced neural stem cells (iNSCs) could protect against cell death after treatment with THP1-derived macrophages. We developed an inflammatory model system with THP1-derived macrophages and cortical neuronal cells and investigated the therapeutic efficacy of iNSC against macrophage-induced inflammation in this model. Apoptosis was confirmed by double immunocytochemistry with NeuN and 4',6-diamidino-2-phenylindole using terminal deoxynucleotidyl transferase-mediated digoxigenin-dUTP-biotin nick-end labeling. Cortical neuronal cells cultured with iNSCs exhibited fewer apoptotic cells than did cultures without iNSCs. The levels of inflammatory cytokines and vascular endothelial growth factor (VEGF) were analyzed by enzyme-linked immunosorbent assay. Cells cultured with iNSCs had lower levels of inflammatory cytokines and higher VEGF levels than those cultured without iNSCs. Western blot analysis for cyclooxygenase-2 (COX-2) showed a significantly lower level of COX-2 in cells cultured with iNSCs than in those cultured without iNSCs. Thus, early iNSCs administration reduced inflammation associated with neurological recovery, and this effect is mediated by COX-2 regulation. Our results suggest that iNSCs have potential therapeutic relevance, because they display strong anti-inflammatory functions that promote neuroprotection thorough the inflammatory response.
Details
- Title: Subtitle
- iNSC suppress macrophage-induced inflammation by repressing COX-2
- Creators
- Jin Hee Kim - Korea UniversityWoong Sun - Korea UniversityDong Wook Han - Konkuk UniversityHong-Joo Moon - Korea University Medical CenterJangbo Lee - Korea Univ, Coll Med, Dept Neurosurg, Seoul 136705, South Korea
- Resource Type
- Journal article
- Publication Details
- In vitro cellular & developmental biology. Animal, Vol.51(2), pp.157-164
- Publisher
- Springer Nature
- DOI
- 10.1007/s11626-014-9816-4
- PMID
- 25248435
- ISSN
- 1071-2690
- eISSN
- 1543-706X
- Number of pages
- 8
- Grant note
- HI12C0337 / Ministry of Health & Welfare, Republic of Korea
- Language
- English
- Date published
- 02/01/2015
- Academic Unit
- Neurosurgery
- Record Identifier
- 9984459629802771
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