Journal article
l- and d- S-nitroso- β, β-dimethylcysteine differentially increase cGMP in cultured vascular smooth muscle cells
European journal of pharmacology, Vol.318(1), pp.47-53
1996
DOI: 10.1016/S0014-2999(96)00719-4
PMID: 9007512
Abstract
We examined the effects of the
l- and
d-isomers of
S-nitroso-
β,
β-dimethylcysteine (
l- and
d-
S-nitrosopenicillamine, 10
−7–10
−5 M) on the guanosine 3′,5′-cyclic monophosphate (cGMP) content of cultured porcine aortic smooth muscle cells and the decomposition of these stereoisomers to nitric oxide (NO).
l-
S-nitrosopenicillamine was a more potent generator of cGMP than
d-
S-nitrosopenicillamine although both stereoisomers equally decomposed to NO. The 10
−7 M concentration of
l- or
d-
S-nitrosopenicillamine did not generate detectable amounts of NO although 10
−7 M
l-
S-nitrosopenicillamine but not
d-
S-nitrosopenicillamine generated significant amounts of cGMP. This study shows that the stereoisomeric configuration of
S-nitrosopenicillamine is an important factor in its biological potency. The data suggest that the extracellular or intracellular generation of NO is not the only mechanism by which this
S-nitrosothiol generates cGMP in vascular smooth muscle.
Details
- Title: Subtitle
- l- and d- S-nitroso- β, β-dimethylcysteine differentially increase cGMP in cultured vascular smooth muscle cells
- Creators
- Mark D Travis - Department of Pharmacology, 2-272 Bowen Science Building, University of Iowa, Iowa City, IA 52242-1109, USALynn L Stoll - Department of Anesthesia, University of Iowa, Iowa City, IA 52242, USAJames N Bates - Department of Anesthesia, University of Iowa, Iowa City, IA 52242, USAStephen J Lewis - Department of Pharmacology, 2-272 Bowen Science Building, University of Iowa, Iowa City, IA 52242-1109, USA
- Resource Type
- Journal article
- Publication Details
- European journal of pharmacology, Vol.318(1), pp.47-53
- Publisher
- Elsevier B.V
- DOI
- 10.1016/S0014-2999(96)00719-4
- PMID
- 9007512
- ISSN
- 0014-2999
- eISSN
- 1879-0712
- Language
- English
- Date published
- 1996
- Academic Unit
- Anesthesia
- Record Identifier
- 9984007185802771
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