Journal article
mRNA vaccines elicit potent neutralization against multiple SARS-CoV-2 Omicron subvariants and other variants of concern
iScience, Vol.25(12), 105690
12/01/2022
DOI: 10.1016/j.isci.2022.105690
PMCID: PMC9711903
PMID: 36471872
Abstract
SARS-CoV-2 variants of concern (VOCs) have shown resistance to vaccines targeting the original virus strain. An mRNA vaccine encoding the spike protein of Omicron BA1 (BA1-S-mRNA) was designed, and its neutralizing activity, with or without the original receptor-binding domain (RBD)-mRNA, was tested against SARS-CoV-2 VOCs. First-dose of BA1-S-mRNA followed by two-boosts of RBD-mRNA elicited potent neutralizing antibodies (nAbs) against pseudotyped and authentic original SARS-CoV-2; pseudotyped Omicron BA1, BA2, BA2.12.1 and BA5 subvariants, and Alpha, Beta, Gamma and Delta VOCs; authentic Omicron BA1 subvariant and Delta VOC. By contrast, other vaccination strategies, including RBD-mRNA first-dose plus BA1-S-mRNA two-boosts, RBD-mRNA or BA1-S-mRNA three-doses, or their combinations, failed to elicit high nAb titers against all of these viruses. Overall, this vaccination strategy was effective for inducing broadly and potent nAbs against multiple SARS-CoV-2 VOCs, particularly Omicron BA5, and may guide the rational design of next-generation mRNA vaccines with greater efficacy against future variants.
Details
- Title: Subtitle
- mRNA vaccines elicit potent neutralization against multiple SARS-CoV-2 Omicron subvariants and other variants of concern
- Creators
- Gang Wang - Georgia State UniversityJuan Shi - Georgia State UniversityAbhishek K. Verma - Department of Microbiology and ImmunologyXiaoqing Guan - Georgia State UniversityStanley Perlman - University of IowaLanying Du - Georgia State University
- Resource Type
- Journal article
- Publication Details
- iScience, Vol.25(12), 105690
- Publisher
- The Author(s)
- DOI
- 10.1016/j.isci.2022.105690
- PMID
- 36471872
- PMCID
- PMC9711903
- ISSN
- 2589-0042
- eISSN
- 2589-0042
- Grant note
- DOI: 10.13039/100000002, name: National Institutes of Health, award: R01AI137472, R01AI139092, R01AI157975
- Language
- English
- Date published
- 12/01/2022
- Academic Unit
- Microbiology and Immunology; Stead Family Department of Pediatrics; Iowa Neuroscience Institute; Infectious Disease (Pediatrics)
- Record Identifier
- 9984321878202771
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