mRNA vaccines elicit potent neutralization against multiple SARS-CoV-2 Omicron subvariants and other variants of concern

Gang Wang; Juan Shi; Abhishek K. Verma; Xiaoqing Guan; Stanley Perlman; Lanying Du

doi:10.1016/j.isci.2022.105690

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mRNA vaccines elicit potent neutralization against multiple SARS-CoV-2 Omicron subvariants and other variants of concern

Journal article

Open access

Peer reviewed

mRNA vaccines elicit potent neutralization against multiple SARS-CoV-2 Omicron subvariants and other variants of concern

Gang Wang, Juan Shi, Abhishek K. Verma, Xiaoqing Guan, Stanley Perlman and Lanying Du

iScience, Vol.25(12), 105690

12/01/2022

DOI: 10.1016/j.isci.2022.105690

PMCID: PMC9711903

PMID: 36471872

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Abstract

SARS-CoV-2 variants of concern (VOCs) have shown resistance to vaccines targeting the original virus strain. An mRNA vaccine encoding the spike protein of Omicron BA1 (BA1-S-mRNA) was designed, and its neutralizing activity, with or without the original receptor-binding domain (RBD)-mRNA, was tested against SARS-CoV-2 VOCs. First-dose of BA1-S-mRNA followed by two-boosts of RBD-mRNA elicited potent neutralizing antibodies (nAbs) against pseudotyped and authentic original SARS-CoV-2; pseudotyped Omicron BA1, BA2, BA2.12.1 and BA5 subvariants, and Alpha, Beta, Gamma and Delta VOCs; authentic Omicron BA1 subvariant and Delta VOC. By contrast, other vaccination strategies, including RBD-mRNA first-dose plus BA1-S-mRNA two-boosts, RBD-mRNA or BA1-S-mRNA three-doses, or their combinations, failed to elicit high nAb titers against all of these viruses. Overall, this vaccination strategy was effective for inducing broadly and potent nAbs against multiple SARS-CoV-2 VOCs, particularly Omicron BA5, and may guide the rational design of next-generation mRNA vaccines with greater efficacy against future variants.

Coronavirus

COVID-19

Neutralizing antibodies

Omicron subvariants

SARS-CoV-2

Variants of concern

Details

Title: Subtitle: mRNA vaccines elicit potent neutralization against multiple SARS-CoV-2 Omicron subvariants and other variants of concern
Creators: Gang Wang - Georgia State University
Juan Shi - Georgia State University
Abhishek K. Verma - Department of Microbiology and Immunology
Xiaoqing Guan - Georgia State University
Stanley Perlman - University of Iowa
Lanying Du - Georgia State University
Resource Type: Journal article
Publication Details: iScience, Vol.25(12), 105690
DOI: 10.1016/j.isci.2022.105690
PMID: 36471872
PMCID: PMC9711903
NLM abbreviation: iScience
ISSN: 2589-0042
eISSN: 2589-0042
Publisher: The Author(s)
Grant note: DOI: 10.13039/100000002, name: National Institutes of Health, award: R01AI137472, R01AI139092, R01AI157975
Language: English
Date published: 12/01/2022
Academic Unit: Microbiology and Immunology; Stead Family Department of Pediatrics; Iowa Neuroscience Institute; Infectious Disease (Pediatrics)
Record Identifier: 9984321878202771

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