microRNA-200c Mitigates Pulpitis and Promotes Dentin Regeneration

Tadkamol Krongbaramee; Chawin Upara; Matthew T. Remy; Long Jiang; Jue Hu; Kittiphoj Tikkhanarak; Bruno Cavalcanti; Hongli Sun; Fabricio B. Teixeira; Liu Hong

doi:10.3390/ijms26146734

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microRNA-200c Mitigates Pulpitis and Promotes Dentin Regeneration

Journal article

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microRNA-200c Mitigates Pulpitis and Promotes Dentin Regeneration

Tadkamol Krongbaramee, Chawin Upara, Matthew T. Remy, Long Jiang, Jue Hu, Kittiphoj Tikkhanarak, Bruno Cavalcanti, Hongli Sun, Fabricio B. Teixeira and Liu Hong

International journal of molecular sciences, Vol.26(14), 6734

07/14/2025

DOI: 10.3390/ijms26146734

PMCID: PMC12296186

PMID: 40724983

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Abstract

MicroRNA (miR)-200c enhances osteogenesis, modulates inflammation, and participates in dentin development. This study was to investigate the beneficial potential of miR-200c in vital pulp therapy (VPT) by mitigating pulpitis and promoting dentin regeneration. We explored the miR-200c variations in inflamed pulp tissues from patients with symptomatic irreversible pulpitis and primary human dental pulp-derived cells (DPCs) challenged with P.g. lipopolysaccharide (Pg-LPS). We further assessed the functions of overexpression of miR-200c on odontogenic differentiation, pulpal inflammation, and dentin regeneration in vitro and in vivo. Our findings revealed a noteworthy downregulation of miR-200c expression in inflamed pulp tissues and primary human DPCs. Through the overexpression of miR-200c via transfecting plasmid DNA (pDNA), we observed a substantial downregulation of proinflammatory cytokines interleukin (IL)-6 and IL-8 in human DPCs. Furthermore, this overexpression significantly enhanced the transcript and protein levels of odontogenic differentiation markers, including Runt-related transcription factor (Runx)2, osteocalcin (OCN), dentin matrix protein (DMP)1, and dentin sialophosphoprotein (DSPP). In a rat model of pulpitis induced by Pg-LPS, we demonstrated notable benefits by local application of pDNA encoding miR-200c delivered by CaCO3-based nanoparticles to reduce pulpal inflammation and promote dentin formation. These results underscore the significant impact of locally applied miR-200c in modulating pulpal inflammation and facilitating dentin repair, showcasing its ability to improve VPT outcomes.

pulpitis

proinflammatory cytokine

odontogenic differentiation

nanoparticles

Details

Title: Subtitle: microRNA-200c Mitigates Pulpitis and Promotes Dentin Regeneration
Creators: Tadkamol Krongbaramee - University of Iowa
Chawin Upara - University of Iowa
Matthew T. Remy - University of Iowa
Long Jiang - University of Iowa
Jue Hu - University of Iowa
Kittiphoj Tikkhanarak - University of Iowa
Bruno Cavalcanti - University of Michigan
Hongli Sun - University of Iowa, Oral and Maxillofacial Surgery
Fabricio B. Teixeira - University of Iowa
Liu Hong - University of Iowa
Resource Type: Journal article
Publication Details: International journal of molecular sciences, Vol.26(14), 6734
DOI: 10.3390/ijms26146734
PMID: 40724983
PMCID: PMC12296186
NLM abbreviation: Int J Mol Sci
ISSN: 1422-0067
eISSN: 1422-0067
Publisher: MDPI
Grant note: National Institute of Dental and Craniofacial Research (NIDCR)National Institutes of Health (NIH)
This research was supported by the National Institute of Dental and Craniofacial Research (NIDCR) (Grant No. R01DE026433 (LH), R01DE029159 (HS), Matthew T. Remy (Grant No. F31DE031153 and T90DE023520) of the National Institutes of Health (NIH).
Language: English
Date published: 07/14/2025
Academic Unit: Roy J. Carver Department of Biomedical Engineering; Endodontics; Prosthodontics; Craniofacial Anomalies Research Center; Oral and Maxillofacial Surgery; Dental Research
Record Identifier: 9984848121402771

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