Journal article
p16INK4A expression is frequently increased in periorbital and ocular squamous lesions
Diagnostic pathology, Vol.10(1), pp.175-175
09/24/2015
DOI: 10.1186/s13000-015-0396-8
PMCID: PMC4581440
PMID: 26400483
Abstract
p16 expression is a well established biomarker of cervical dysplasia and carcinoma arising from high risk human papilloma virus infection. Increased p16 expression is also seen in squamous neoplasms arising at other sites, including head, neck, and oropharyngeal tract. Squamous lesions are also frequently encountered at ocular surface and peri-orbital skin sites, but the prevalence of increased p16 expression in these lesions has been poorly studied.
We retrospectively surveyed 13 ocular surface and 16 orbital squamous lesions biopsied at UC San Diego Healthcare System and VA San Diego Healthcare System for p16 expression by immunohistochemistry. These cases included ocular surface lesions with diagnoses of conjunctival intraepithelial neoplasm (CIN) and squamous cell carcinoma in situ. Peri-orbital eyelid biopsies included lesions with diagnoses of SCCis and invasive squamous cell carcinoma. We performed multivariate logistic regression, followed by student's T-test or Fisher's exact test to determine if there were statistically significant associations between p16 immunoreactivity and patient age, gender, diagnosis, and ethnicity. Statistical significance was defined as p < 0.05.
We found an unexpectedly large prevalence of strong nuclear and cytoplasmic p16 immunoreactivity in our cases. Almost all of the ocular surface squamous lesions were diffusely positive for p16 expression (12/13). All of the periorbital lesions showed diffuse p16 immunoreactivity (16/16). Altogether, 28/29 lesions tested showed strong and diffuse p16 expression. We found no statistically significant correlation between p16 expression and patient age, gender, ethnicity, or diagnosis. In 6 of the peri-orbital biopsies, we had sufficient tissue to assess high-risk HPV expression by in situ hybridization. Interestingly, all of these cases were negative for HPV, despite strong p16 expression.
Strong p16 expression was observed in virtually all of the ocular surface and peri-orbital squamous neoplasms in our study. The relationship between p16 expression and HPV infection in ocular surface and peri-orbital sites requires further investigation.
Details
- Title: Subtitle
- p16INK4A expression is frequently increased in periorbital and ocular squamous lesions
- Creators
- Peter J Kobalka - Department of Pathology, School of Medicine, UC San Diego, 9500 Gilman Dr., La Jolla, CA, 92093-0612, USA. pkobalka@ucsd.eduJean-Paul Abboud - Shiley Eye Center and Department of Ophthalmology, University of California San Diego and VA San Diego Healthcare System, San Diego, CA, USA. jabboud@ucsd.eduXiaoyan Liao - Department of Pathology, University of California San Diego and VA San Diego Healthcare System, San Diego, CA, USA. x1liao@ucsd.eduKarra Jones - Department of Pathology, University of California San Diego and VA San Diego Healthcare System, San Diego, CA, USA. kaj014@ucsd.edBradford W Lee - Shiley Eye Center and Department of Ophthalmology, University of California San Diego and VA San Diego Healthcare System, San Diego, CA, USA. bwl010@ucsd.eduBobby S Korn - Shiley Eye Center and Department of Ophthalmology, University of California San Diego and VA San Diego Healthcare System, San Diego, CA, USA. bkorn@ucsd.eduDon O Kikkawa - Shiley Eye Center and Department of Ophthalmology, University of California San Diego and VA San Diego Healthcare System, San Diego, CA, USA. dkikkawa@ucsd.eduJonathan H Lin - Shiley Eye Center and Department of Ophthalmology, University of California San Diego and VA San Diego Healthcare System, San Diego, CA, USA. jlin@ucsd.edu
- Resource Type
- Journal article
- Publication Details
- Diagnostic pathology, Vol.10(1), pp.175-175
- DOI
- 10.1186/s13000-015-0396-8
- PMID
- 26400483
- PMCID
- PMC4581440
- NLM abbreviation
- Diagn Pathol
- ISSN
- 1746-1596
- eISSN
- 1746-1596
- Publisher
- England
- Grant note
- I01 BX002284 / BLRD VA
- Language
- English
- Date published
- 09/24/2015
- Academic Unit
- Pathology
- Record Identifier
- 9984047644902771
Metrics
12 Record Views