p75NTR and sortilin increase after facial nerve injury

Matthew J Provenzano; Ningyong Xu; Mark R Ver Meer; J Jason Clark; Marlan R Hansen

doi:10.1097/MLG.0b013e31814b8d9f

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p75NTR and sortilin increase after facial nerve injury

Journal article

Peer reviewed

p75NTR and sortilin increase after facial nerve injury

Matthew J Provenzano, Ningyong Xu, Mark R Ver Meer, J Jason Clark and Marlan R Hansen

The Laryngoscope, Vol.118(1), pp.87-93

01/2008

DOI: 10.1097/MLG.0b013e31814b8d9f

PMID: 18090258

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Abstract

After axotomy, Schwann cells (SCs), required for successful nerve regeneration, undergo a number of cellular changes including dedifferentiation, proliferation, expression of molecules that support axon growth, and apoptosis. This study investigated the role of p75, sortilin, and proneurotrophins in SC survival after facial nerve (FN) axotomy. Preliminary animal study. With use of FN SCs, expression of p75 and its coreceptor sortilin were quantified by immunofluorescence on days 12, 22, and 52 after axotomy in vivo and by Western blot in vitro. Contralateral FNs served as a control. SC apoptosis was detected by terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL). To verify a causative role for p75 in FN SC death, cultured FN SCs were treated with pro-nerve growth factor (NGF), and apoptosis was determined by TUNEL. Expression of p75 and sortilin increased in FN SCs distal (P < .05) to the axotomy compared with the contralateral controls for all time points. SC apoptosis also significantly increased in the distal segment compared with the contralateral and proximal portions (P < .05). ProNGF, a p75 ligand, increased apoptosis and p75 expression in primary FN SC cultures. FN axotomy increases p75 and sortilin expression in SCs, which correlates with increased apoptosis. These findings suggest roles for p75 and sortilin in SC loss after FN injury. Sortilin is a novel target in promoting FN healing after injury.

Membrane Glycoproteins - analysis

Apoptosis - drug effects

Nerve Tissue Proteins - analysis

Receptors, Nerve Growth Factor - analysis

Receptors, Nerve Growth Factor - physiology

Adaptor Proteins, Vesicular Transport

Nerve Growth Factors - pharmacology

Axotomy

Membrane Glycoproteins - physiology

Cell Death - drug effects

Cell Survival - physiology

Schwann Cells - drug effects

In Situ Nick-End Labeling

Cell Survival - drug effects

Nerve Tissue Proteins - physiology

Cells, Cultured

Gene Expression Regulation

Axons - drug effects

Rats

Schwann Cells - pathology

Rats, Sprague-Dawley

Blotting, Western

Nerve Degeneration - pathology

Protein Precursors - pharmacology

Animals

Axons - pathology

Fluorescent Antibody Technique

Facial Nerve Injuries - pathology

Apoptosis - physiology

Details

Title: Subtitle: p75NTR and sortilin increase after facial nerve injury
Creators: Matthew J Provenzano - Department of Otolaryngology-Head and Neck Surgery, University of Iowa, 200 Hawkins Drive, Iowa City, IA 52242, U.S.A
Ningyong Xu
Mark R Ver Meer
J Jason Clark
Marlan R Hansen
Resource Type: Journal article
Publication Details: The Laryngoscope, Vol.118(1), pp.87-93
Publisher: United States
DOI: 10.1097/MLG.0b013e31814b8d9f
PMID: 18090258
ISSN: 0023-852X
eISSN: 1531-4995
Grant note: T32 DC000040 / NIDCD NIH HHS
Language: English
Date published: 01/2008
Academic Unit: Molecular Physiology and Biophysics; Neurosurgery; Otolaryngology
Record Identifier: 9984007195702771

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