Journal article
ras Gene Mutations in Salivary Gland Tumors
Archives of pathology & laboratory medicine (1976), Vol.124(6), pp.836-839
06/01/2000
DOI: 10.5858/2000-124-0836-RGMISG
PMID: 10835516
Abstract
Abstract Objective.—To assess the prevalence of activating mutations in K-ras and H-ras genes in salivary gland tumors with ductal or acinar differentiation and to evaluate their potential correlation with clinical parameters. Design.—Paraffin-embedded tissue samples of salivary gland carcinomas were investigated by the application of a direct sequence analysis procedure with automated DNA sequencing of polymerase chain reaction–amplified ras sequences. Setting.—Tertiary care teaching hospital. Patients.—Twenty-four patients with salivary gland carcinoma were surgically treated. Nine had adenocarcinoma, 1 had adenosquamous carcinoma, 11 had mucoepidermoid carcinoma, and 3 had acinic cell carcinoma. Results.—Point mutations were detected in 7 (29%) of the 24 carcinomas examined. The K-ras gene was mutated in only 2 samples (8%): a GGC-to-ATC mutation at codon 13 in an adenocarcinoma and a GGC-to-GTC transversion mutation at codon 13 in a mucoepidermoid carcinoma. Five (21%) harbored H-ras mutations: 4 contained a GGC-to-GTC transversion mutation at codon 12 and 1 had 2 distinct mutations, the same G-to-T at codon 12 as was shown in the other cases and a GGT-to-GGA heterozygous mutation at codon 13. All the H-ras mutations were in the group of mucoepidermoid carcinoma lesions (45%; 5/11). Conclusion.—Our data suggest that K-ras gene alteration is probably not an important factor in the oncogenesis of human salivary gland tumors. However, mutational activation of the H-ras gene appears to play a role in the development and/or progression of salivary gland mucoepidermoid carcinomas.
Details
- Title: Subtitle
- ras Gene Mutations in Salivary Gland Tumors
- Creators
- Jinyoung Yoo - From the Department of Pathology, Catholic University, St Vincent Hospital, Suwon, Republic of Korea (Dr Yoo), and Department of Pathology, University of Iowa, Iowa City (Dr Robinson)Robert A Robinson - Reprints: Robert A. Robinson, MD, PhD, Department of Pathology, University of Iowa Hospitals and Clinics, 200 Hawkins Dr, 5239B RCP, Iowa City, IA 52242-1009 (e-mail: robert-a-robinson@uiowa.edu)., From the Department of Pathology, Catholic University, St Vincent Hospital, Suwon, Republic of Korea (Dr Yoo), and Department of Pathology, University of Iowa, Iowa City (Dr Robinson)
- Resource Type
- Journal article
- Publication Details
- Archives of pathology & laboratory medicine (1976), Vol.124(6), pp.836-839
- DOI
- 10.5858/2000-124-0836-RGMISG
- PMID
- 10835516
- ISSN
- 0003-9985
- eISSN
- 1543-2165
- Language
- English
- Date published
- 06/01/2000
- Academic Unit
- Oral Pathology, Radiology and Medicine; Pathology
- Record Identifier
- 9984046914802771
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