von Willebrand factor interacts with malignant hematopoietic cell lines: evidence for the presence of specific binding sites and modification of von Willebrand factor structure and function

C M Floyd; K Irani; P D Kind; C M Kessler

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von Willebrand factor interacts with malignant hematopoietic cell lines: evidence for the presence of specific binding sites and modification of von Willebrand factor structure and function

Journal article

Peer reviewed

von Willebrand factor interacts with malignant hematopoietic cell lines: evidence for the presence of specific binding sites and modification of von Willebrand factor structure and function

C M Floyd, K Irani, P D Kind and C M Kessler

The Journal of laboratory and clinical medicine, Vol.119(5), pp.467-476

05/1992

PMID: 1583402

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Abstract

von Willebrand factor (vWf) mediates the adherence of platelets to exposed vascular subendothelium. During hematogenous metastasis in certain model systems, platelets are also deposited at endothelial surfaces in heterotypic aggregates with tumor cells. The role of vWf in this metastatic event was investigated by examining the interaction between purified iodine 125-labeled vWf and the human tumor cell lines, U937 and CA46. vWf-tumor cell binding was specific, partially reversible, and saturable at 4 degrees C. At 37 degrees C, however, U937 cell binding sites could not be saturated, suggesting endocytosis as a possible mechanism of interaction. When constant amounts of vWf were added, interactions with tumor cells were complete after 60 minutes, but rapidly dissociated over the next 2 hours. With the addition of specific proteinase inhibitors, vWf remained in stable association with tumor cells throughout a 3-hour time course. Loss of vWf antigen in supernatant samples complemented the 125I-labeled vWf bound directly to tumor cells. However, disproportionately large reductions in vWf functional activity were observed and correlated with alterations in multimeric structure. These data suggest an initial binding of vWf to some tumor cells, followed by a time-dependent loss of structural and functional integrity. VWf may contribute to tumor cell arrest within the microvasculature and may influence other tumor cell-subendothelial interactions as the adhesive function of vWf is subsequently modified.

Amino Acid Sequence

Cell Survival - drug effects

Humans

Molecular Sequence Data

Hematopoietic Stem Cells - metabolism

Structure-Activity Relationship

Oligopeptides - metabolism

Biological Transport

von Willebrand Factor - chemistry

Protein Binding

Tumor Cells, Cultured

In Vitro Techniques

Binding Sites

von Willebrand Factor - metabolism

Details

Title: Subtitle: von Willebrand factor interacts with malignant hematopoietic cell lines: evidence for the presence of specific binding sites and modification of von Willebrand factor structure and function
Creators: C M Floyd - Division of Hematology/Oncology, George Washington University Medical Center, Washington, D.C. 20037
K Irani
P D Kind
C M Kessler
Resource Type: Journal article
Publication Details: The Journal of laboratory and clinical medicine, Vol.119(5), pp.467-476
PMID: 1583402
NLM abbreviation: J Lab Clin Med
ISSN: 0022-2143
eISSN: 1532-6543
Publisher: Elsevier; United States
Language: English
Date published: 05/1992
Academic Unit: Cardiovascular Medicine; Radiation Oncology; Fraternal Order of Eagles Diabetes Research Center; Internal Medicine
Record Identifier: 9984047991202771

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