α Subunit isoform influences GABA A receptor modulation by propofol

M.D Krasowski; S.M O'Shea; C.E.M Rick; P.J Whiting; K.L Hadingham; C Czajkowski; N.L Harrison

doi:10.1016/S0028-3908(97)00074-9

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α Subunit isoform influences GABA A receptor modulation by propofol

Journal article

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Peer reviewed

α Subunit isoform influences GABA A receptor modulation by propofol

M.D Krasowski, S.M O'Shea, C.E.M Rick, P.J Whiting, K.L Hadingham, C Czajkowski and N.L Harrison

Neuropharmacology, Vol.36(7), pp.941-949

1997

DOI: 10.1016/S0028-3908(97)00074-9

PMID: 9257938

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https://www.ncbi.nlm.nih.gov/pmc/articles/2857729View

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Abstract

We have investigated the role of the α subunit in the modulation of γ-aminobutyric acid type A (GABA A) receptors by the general anesthetic propofol, using whole-cell patch clamp recordings made from distinct stable fibroblast cell lines which expressed only α 1 β 3 γ 2 or α 6 β 3 γ 2 GABA A receptors. At clinically relevant anesthetic concentrations, propofol potentiated submaximal GABA currents in α 1 β 3 γ 2 receptors to a far greater degree than those in α 6 β 3 γ 2 receptors. The a subunit influenced the efficacy of propofol for modulation, but not its potency. In contrast, direct gating of the ion channel by propofol, in the absence of GABA, was significantly larger in the α 6 than the α 1 containing receptors. The potentiation of submaximal GABA by trichloroethanol, and the potentiation and direct gating by methohexital was also studied, and showed the same relative trends as propofol.

GABA

trichloroethanol

GABA A receptor

methohexital

propofol

anesthesia

Details

Title: Subtitle: α Subunit isoform influences GABA A receptor modulation by propofol
Creators: M.D Krasowski - Department of Anesthesia and Critical Care and the Department of Pharmacological and Physiological Sciences, University of Chicago, Chicago, IL 60637, U.S.A
S.M O'Shea - Department of Anesthesia and Critical Care and the Department of Pharmacological and Physiological Sciences, University of Chicago, Chicago, IL 60637, U.S.A
C.E.M Rick - Department of Anesthesia and Critical Care and the Department of Pharmacological and Physiological Sciences, University of Chicago, Chicago, IL 60637, U.S.A
P.J Whiting - Merck, Sharpe, and Dohme Research Laboratories, Terlings Park, Harlow, Essex, U.K
K.L Hadingham - Merck, Sharpe, and Dohme Research Laboratories, Terlings Park, Harlow, Essex, U.K
C Czajkowski - Department of Neurophysiology, University of Wisconsin, Madison, WI 53706, U.S.A
N.L Harrison - Department of Anesthesia and Critical Care and the Department of Pharmacological and Physiological Sciences, University of Chicago, Chicago, IL 60637, U.S.A
Resource Type: Journal article
Publication Details: Neuropharmacology, Vol.36(7), pp.941-949
DOI: 10.1016/S0028-3908(97)00074-9
PMID: 9257938
NLM abbreviation: Neuropharmacology
ISSN: 0028-3908
eISSN: 1873-7064
Publisher: Elsevier Ltd
Language: English
Date published: 1997
Academic Unit: Pathology
Record Identifier: 9984047645402771

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