Journal article
β-Adrenergic agonists regulate Na-K-ATPase via p70S6k
American journal of physiology. Lung cellular and molecular physiology, Vol.285(4), pp.L802-L807
10/2003
DOI: 10.1152/ajplung.00266.2002
PMID: 12704019
Abstract
We have recently reported that the β-adrenergic agonist isoproterenol regulates the alveolar epithelial cell Na-K-ATPase via MAPK/extracellular signal-regulated kinase and rapamycin-sensitive pathways. Here we report that isoproterenol phosphorylated the protein S6 kinase (p70S6k) in alveolar epithelial cells, which was inhibited by both rapamycin and the MEK1/2 inhibitor U-0126. In alveolar epithelial cells transfected with a p70S6k dominant negative construct, isoproterenol did not increase Na-K-ATPase total protein expression, whereas in cells transfected with a rapamycin-resistant mutant, the isoproterenol-mediated increase in Na-K-ATPase was not prevented by rapamycin. Accordingly, we provide here first evidence that isoproterenol regulates Na-K-ATPase via p70S6k in alveolar epithelial cells.
Details
- Title: Subtitle
- β-Adrenergic agonists regulate Na-K-ATPase via p70S6k
- Creators
- Liuska Pesce - Division of Pulmonary and Critical Care Medicine, Feinberg School of Medicine, Northwestern University, Chicago, Illinois 60611Alejandro Comellas - Division of Pulmonary and Critical Care Medicine, Feinberg School of Medicine, Northwestern University, Chicago, Illinois 60611Jacob I Sznajder - Division of Pulmonary and Critical Care Medicine, Feinberg School of Medicine, Northwestern University, Chicago, Illinois 60611
- Resource Type
- Journal article
- Publication Details
- American journal of physiology. Lung cellular and molecular physiology, Vol.285(4), pp.L802-L807
- DOI
- 10.1152/ajplung.00266.2002
- PMID
- 12704019
- ISSN
- 1040-0605
- eISSN
- 1522-1504
- Language
- English
- Date published
- 10/2003
- Academic Unit
- Pulmonary, Critical Care, and Occupational Medicine; Endocrinology and Diabetes; ICTS; Stead Family Department of Pediatrics; Internal Medicine
- Record Identifier
- 9984093359102771
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