β-Agonists regulate Na,K-ATPase via novel MAPK/ERK and rapamycin-sensitive pathways

Liuska Pesce; Carmen Guerrero; Alejandro Comellas; Karen M Ridge; Jacob I Sznajder

doi:10.1016/S0014-5793(00)02298-5

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β-Agonists regulate Na,K-ATPase via novel MAPK/ERK and rapamycin-sensitive pathways

Journal article

Peer reviewed

β-Agonists regulate Na,K-ATPase via novel MAPK/ERK and rapamycin-sensitive pathways

Liuska Pesce, Carmen Guerrero, Alejandro Comellas, Karen M Ridge and Jacob I Sznajder

FEBS letters, Vol.486(3), pp.310-314

2000

DOI: 10.1016/S0014-5793(00)02298-5

PMID: 11119725

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Abstract

We studied whether the β-adrenergic agonist, isoproterenol (ISO), regulates Na,K-ATPase in alveolar epithelial cells (AEC) via a mitogen-activated protein kinase (MAPK)/extracellular signaling related kinase (ERK) dependent pathway. ISO increased ERK activity in AEC by 10 min via a β-adrenergic receptor, protein kinase A (PKA)-dependent mechanism. Activation of the MAPK pathway by ISO, resulted in increased Na,K-ATPase β1 and α1 subunit protein abundance in whole cell lysates, which resulted in functional Na,K-ATPases at the basolateral membranes. ISO did not change the α1 or β1 mRNA steady state levels, but rapamycin, the inhibitor of the mammalian target of rapamycin, also blocked the ISO-mediated increase in Na,K-ATPase total protein abundance, suggesting a posttranscriptional regulation. We conclude that ISO, regulates the Na,K-ATPase in AEC via PKA, ERK and rapamycin-sensitive mechanisms.

Protein kinase A

Isoproterenol

cAMP

Lung

Mammalian target of rapamycin

Details

Title: Subtitle: β-Agonists regulate Na,K-ATPase via novel MAPK/ERK and rapamycin-sensitive pathways
Creators: Liuska Pesce - Division of Pulmonary and Critical Care Medicine, Northwestern University, 300 E. Superior St., Tarry Building 14-707, Chicago, IL 60611, USA
Carmen Guerrero - Division of Pulmonary and Critical Care Medicine, Northwestern University, 300 E. Superior St., Tarry Building 14-707, Chicago, IL 60611, USA
Alejandro Comellas - Division of Pulmonary and Critical Care Medicine, Northwestern University, 300 E. Superior St., Tarry Building 14-707, Chicago, IL 60611, USA
Karen M Ridge - Division of Pulmonary and Critical Care Medicine, Northwestern University, 300 E. Superior St., Tarry Building 14-707, Chicago, IL 60611, USA
Jacob I Sznajder - Division of Pulmonary and Critical Care Medicine, Northwestern University, 300 E. Superior St., Tarry Building 14-707, Chicago, IL 60611, USA
Resource Type: Journal article
Publication Details: FEBS letters, Vol.486(3), pp.310-314
Publisher: Elsevier B.V
DOI: 10.1016/S0014-5793(00)02298-5
PMID: 11119725
ISSN: 0014-5793
eISSN: 1873-3468
Language: English
Date published: 2000
Academic Unit: Pulmonary, Critical Care, and Occupational Medicine; Endocrinology and Diabetes; ICTS; Stead Family Department of Pediatrics; Internal Medicine
Record Identifier: 9984093350002771

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