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β Subunit Heterogeneity in N-type Ca2+ Channels (∗)
Journal article   Open access   Peer reviewed

β Subunit Heterogeneity in N-type Ca2+ Channels (∗)

Victoria E.S Scott, Michel De Waard, Hongyan Liu, Christina A Gurnett, David P Venzke, Vanda A Lennon and Kevin P Campbell
The Journal of biological chemistry, Vol.271(6), pp.3207-3212
02/09/1996
DOI: 10.1074/jbc.271.6.3207
PMID: 8621722
url
https://doi.org/10.1074/jbc.271.6.3207View
Published (Version of record) Open Access

Abstract

The β subunit of the voltage-dependent Ca2+ channel is a cytoplasmic protein that interacts directly with an α1 subunit, thereby modulating the biophysical properties of the channel. Herein, we demonstrate that the α1B subunit of the N-type Ca2+ channel associates with several different β subunits. Polyclonal antibodies specific for three different β subunits immunoprecipitated 125I-ω-conotoxin GVIA binding from solubilized rabbit brain membranes. Enrichment of the N-type Ca2+ channels with an α1B subunit-specific monoclonal antibody showed the association of β1b, β3, and β4 subunits. Protein sequencing of tryptic peptides of the 57-kDa component of the purified N-type Ca2+ channel confirmed the presence of the β3 and β4 subunits. Each of the β subunits bound to the α1B subunit interaction domain with similar high affinity. Thus, our data demonstrate important heterogeneity in the β subunit composition of the N-type Ca2+ channels, which may be responsible for some of the diverse kinetic properties recorded from neurons.

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