Worry and FRET: ROS Production Leads to Fluorochrome Tandem Degradation and impairs Interpretation of Flow Cytometric Results

Letter/Communication

Open access

Isaac J Jensen, Patrick W McGonagill, Mitchell N Lefebvre, Thomas S Griffith, John T Harty and Vladimir P Badovinac

Immunity (Cambridge, Mass.), Vol.52(3), pp.419-421

03/17/2020

DOI: 10.1016/j.immuni.2020.02.003

PMCID: PMC7251922

PMID: 32187510

Files and links (1)

url

https://doi.org/10.1016/j.immuni.2020.02.003View

Published (Version of record) Open Access

Animals

Benzothiazoles - chemistry

Benzothiazoles - metabolism

Carbocyanines - chemistry

Carbocyanines - metabolism

Flow Cytometry - methods

Fluorescence Resonance Energy Transfer - methods

Fluorescent Dyes - chemistry

Fluorescent Dyes - metabolism

Granulocytes - chemistry

Granulocytes - metabolism

Humans

Mercaptoethanol - chemistry

Mercaptoethanol - metabolism

Phycocyanin - chemistry

Phycocyanin - metabolism

Reactive Oxygen Species - metabolism

Reproducibility of Results

Title: Subtitle: Worry and FRET: ROS Production Leads to Fluorochrome Tandem Degradation and impairs Interpretation of Flow Cytometric Results
Creators: Isaac J Jensen - University of Iowa
Patrick W McGonagill - University of Iowa
Mitchell N Lefebvre - University of Iowa
Thomas S Griffith - University of Minnesota
John T Harty - University of Iowa
Vladimir P Badovinac - University of Iowa
Resource Type: Letter/Communication
Publication Details: Immunity (Cambridge, Mass.), Vol.52(3), pp.419-421
DOI: 10.1016/j.immuni.2020.02.003
PMID: 32187510
PMCID: PMC7251922
ISSN: 1074-7613
eISSN: 1097-4180
Grant note: I01 BX001324 / BLRD VA R21 AI147064 / NIAID NIH HHS R01 AI114543 / NIAID NIH HHS R35 GM134880 / NIGMS NIH HHS R01 AI100527 / NIAID NIH HHS T32 GM007337 / NIGMS NIH HHS R01 GM113961 / NIGMS NIH HHS R01 AI042767 / NIAID NIH HHS R21 AI042767 / NIAID NIH HHS T32 AI007511 / NIAID NIH HHS R37 AI042767 / NIAID NIH HHS T32 AI007485 / NIAID NIH HHS R01 AI085515 / NIAID NIH HHS R01 GM115462 / NIGMS NIH HHS
Language: English
Date published: 03/17/2020
Academic Unit: Microbiology and Immunology; Pathology; Surgery
Record Identifier: 9984181070202771

15 Record Views