Methods used for lipidomics of INS-1 cells performed at Metabolomics and lipidomics core facility, at the University of Utha (2026)

Yumi Imai

doi:10.17077/misc.006810

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Methods used for lipidomics of INS-1 cells performed at Metabolomics and lipidomics core facility, at the University of Utha (2026)

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Methods used for lipidomics of INS-1 cells performed at Metabolomics and lipidomics core facility, at the University of Utha (2026)

Yumi Imai

University of Iowa

07/28/2026

DOI: 10.17077/misc.006810

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Abstract

Lipidomics of complex lipids and acylcarnitine were performed at Metabolomics and lipidomics Core within University of Utha per their protocol. INS-1 cells on 6 well plate was washed 2X with PBS, trypinized, centrifuged, and snap frozen in liquid nitrogen. Lipids were processed using a modified Matyash lipid extraction workflow and protein content was measured separately by BCA assay to normalize lipid abundance to sample input. This document describes detail protocol utilized for lipidomics.

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Title: Subtitle: Methods used for lipidomics of INS-1 cells performed at Metabolomics and lipidomics core facility, at the University of Utha (2026)
Creators: Yumi Imai - University of Iowa, Endocrinology and Metabolism
Resource Type: Other
DOI: 10.17077/misc.006810
Publisher: University of Iowa
Number of pages: 3 pages
Language: English
Date published: 07/28/2026
Academic Unit: Endocrinology and Metabolism; Internal Medicine
Record Identifier: 9985182691202771

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