Disclosed are methods for the preparation of polyclonal and monoclonal antibodies which bind specifically to a 43 kDa dystrophin-associated. The molecular weight of the 43 kDa protein is determined by electrophoretic separation under denaturing conditions, followed by transfer to a solid support and staining with wheat germ agglutinin. The method includes a step in which the peptide PKNMTPYRSPPPYVP (SEQ ID NO: 15) is administered to stimulate an immune response. Also disclosed are polyclonal and monoclonal antibodies which bind specifically to the 43 kDa dystrophin-associated protein.
Patent
Polyclonal and monoclonal antibodies against a 43 KDA dystrophin associated protein
United States Patent and Trademark Office
11/11/1997
Abstract
Details
- Title: Subtitle
- Polyclonal and monoclonal antibodies against a 43 KDA dystrophin associated protein
- Creators
- Kevin P Campbell (Inventor)Oxana B Ibraghimov (Inventor)James M Ervasti (Inventor)Cynthia J Leveille (Inventor)
- Contributors
- University of Iowa Research Foundation (Iowa City, IA, US) (Assignee)
- Resource Type
- Patent
- Publisher
- United States Patent and Trademark Office; United States
- Patent
- US Patent 5,686,073; Published; 08/483,278; 06/07/1995; 424/185.1; 435/70.21, 435/452, 436/518, 530/326, 530/388.85, 530/389.1
- Number of pages
- 41 pages
- Language
- English
- Date published
- 11/11/1997
- Academic Unit
- Iowa Neuroscience Institute; UI Research Foundation
- Record Identifier
- 9983762086602771
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