Purification of heparinase I, II, and III from Flavobacterium heparinum

Ramnath Sasisekharan; Daniel L Lohse; Charles L Cooney; Robert J Linhardt; Robert S Langer

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Purification of heparinase I, II, and III from Flavobacterium heparinum

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Purification of heparinase I, II, and III from Flavobacterium heparinum

Ramnath Sasisekharan, Daniel L Lohse, Charles L Cooney, Robert J Linhardt and Robert S Langer

United States Patent and Trademark Office

02/14/1995

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Abstract

A purified heparinase I, II and III free of lyase activity and each having a molecular weight of 42,800 84,100, 70,800, respectively, are produced by culturing Flavobacterium heparinum. The kinetic properties of the heparinases have been determined as well as the conditions to optimize their activity and stability.

Details

Title: Subtitle: Purification of heparinase I, II, and III from Flavobacterium heparinum
Creators: Ramnath Sasisekharan (Inventor)
Daniel L Lohse (Inventor)
Charles L Cooney (Inventor)
Robert J Linhardt (Inventor)
Robert S Langer (Inventor)
Contributors: Massachusetts Institute of Technology (Cambridge, MA) (Assignee)
University of Iowa Research Foundation (Iowa City, IA, US) (Assignee)
Resource Type: Patent
Publisher: United States Patent and Trademark Office; United States
Patent: US Patent 5,389,539; United States Patent and Trademark Office (United States, Alexandria) - USPTO; Published; 07/983,367; 11/30/1992
Number of pages: 19 pages
Language: English
Date published: 02/14/1995
Academic Unit: UI Research Foundation
Record Identifier: 9983761991002771

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