A Method for Analyzing AFM Force Mapping Data Obtained from Soft Tissue Cryosections

Cydney A Wong; Nina Sara Fraticelli Guzmán; A Thomas Read; Adam Hedberg-Buenz; Michael G Anderson; Andrew J Feola; Todd Sulchek; C Ross Ethier

A Method for Analyzing AFM Force Mapping Data Obtained from Soft Tissue Cryosections

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A Method for Analyzing AFM Force Mapping Data Obtained from Soft Tissue Cryosections

Cydney A Wong, Nina Sara Fraticelli Guzmán, A Thomas Read, Adam Hedberg-Buenz, Michael G Anderson, Andrew J Feola, Todd Sulchek and C Ross Ethier

bioRxiv : the preprint server for biology

11/13/2023

PMCID: PMC10680563

PMID: 38014311

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url

https://doi.org/10.1101/2023.11.08.566263View

Preprint (Author's original)This preprint has not been evaluated by subject experts through peer review. Preprints may undergo extensive changes and/or become peer-reviewed journal articles. Open Access

Abstract

Atomic force microscopy (AFM) is a valuable tool for assessing mechanical properties of biological samples, but interpretations of measurements on whole tissues can be difficult due to the tissue's highly heterogeneous nature. To overcome such difficulties and obtain more robust estimates of tissue mechanical properties, we describe an AFM force mapping and data analysis pipeline to characterize the mechanical properties of cryosectioned soft tissues. We assessed this approach on mouse optic nerve head and rat trabecular meshwork, cornea, and sclera. Our data show that the use of repeated measurements, outlier exclusion, and log-normal data transformation increases confidence in AFM mechanical measurements, and we propose that this methodology can be broadly applied to measuring soft tissue properties from cryosections.

Details

Title: Subtitle: A Method for Analyzing AFM Force Mapping Data Obtained from Soft Tissue Cryosections
Creators: Cydney A Wong - Emory University
Nina Sara Fraticelli Guzmán - Georgia Institute of Technology
A Thomas Read - Emory University
Adam Hedberg-Buenz - University of Iowa
Michael G Anderson - Department of Molecular Physiology & Biophysics, University of Iowa, Iowa City, IA
Andrew J Feola - Atlanta VA Medical Center
Todd Sulchek - Georgia Institute of Technology
C Ross Ethier - Emory University
Resource Type: Preprint
Publication Details: bioRxiv : the preprint server for biology
Publisher: United States
PMID: 38014311
PMCID: PMC10680563
Grant note: R01 EY031710 / NEI NIH HHS IK2 RX002342 / RRD VA P30 EY006360 / NEI NIH HHS R01 EY030871 / NEI NIH HHS T32 EY007092 / NEI NIH HHS R21 EY031598 / NEI NIH HHS T32 GM145735 / NIGMS NIH HHS
Language: English
Date posted: 11/13/2023
Academic Unit: Molecular Physiology and Biophysics; Ophthalmology and Visual Sciences
Record Identifier: 9984530557302771

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