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A Zur-dependent regulatory RNA involved in maintaining zinc homeostasis in Staphylococcus aureus
Preprint   Open access

A Zur-dependent regulatory RNA involved in maintaining zinc homeostasis in Staphylococcus aureus

Mathilde Charbonnier, Samuel Probst-Lotze, Hugo Racine, Jana N. Radin, Gustavo Rios-Delgado, Hannah M. Laster, Maximilian P. Kohl, Rafał Mazgaj, Marion Blum, Virginie Marchand, …
bioRxiv
Cold Spring Harbor Laboratory
08/23/2025
DOI: 10.1101/2025.08.23.671911
PMCID: PMC12393559
PMID: 40894724
url
https://doi.org/10.1101/2025.08.23.671911View
Preprint (Author's original) This preprint has not been evaluated by subject experts through peer review. Preprints may undergo extensive changes and/or become peer-reviewed journal articles. Open Access

Abstract

Small regulatory RNAs (sRNAs) are key drivers of bacterial adaptation to environmental fluctuations, including iron and manganese restriction imposed by the host. This study explored the repertoire of sRNAs produced by the human pathogen Staphylococus aureus in response to metal limitation. Two sRNAs, S1077 and ZinS (RsaX20), regulated by zinc (Zn) availability, were identified. Further investigations revealed that, similar to the cnt operon from which it derives, S1077 synthesis is controlled by the transcription factors Zur and Fur. In contrast, zinS transcription is solely repressed by Zur. Amongst the ZinS targets are several Zn-dependent enzymes, such as the alcohol dehydrogenase Adh, whose synthesis is negatively regulated by ZinS. Loss of ZinS does not alter staphylococcal metal accumulation, suggesting a role in a Zn-sparing response. Remarkably, zinS also encodes a small peptide, ZinP. Genomic analysis suggests that the regulatory portion of ZinS emerged from the 3’ untranslated region of zinP in S. aureus and closely related species after horizontal gene transfer from phylogenetically distant organisms. All our findings demonstrate that sRNAs also facilitate bacterial adaptation to Zn limitation, and that genetic exchange and subsequent neofunctionalization have enabled S. aureus to adapt to metal-restricted environments.

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