CaCO 3 Nanoparticles Delivering MicroRNA-200c Suppress Oral Squamous Cell Carcinoma

Qiong J Ding; Matthew T. Remy; Chawin Upara; Jue Hu; Amanda J. Haes; Emily Lanzel; Hongli Sun; Marisa R. Buchakjian; Liu Hong

doi:10.1101/2023.10.05.561110

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CaCO 3 Nanoparticles Delivering MicroRNA-200c Suppress Oral Squamous Cell Carcinoma

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CaCO 3 Nanoparticles Delivering MicroRNA-200c Suppress Oral Squamous Cell Carcinoma

Qiong J Ding, Matthew T. Remy, Chawin Upara, Jue Hu, Amanda J. Haes, Emily Lanzel, Hongli Sun, Marisa R. Buchakjian and Liu Hong

bioRxiv

Cold Spring Harbor Laboratory

10/05/2023

DOI: 10.1101/2023.10.05.561110

PMCID: PMC10592969

PMID: 37873146

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https://doi.org/10.1101/2023.10.05.561110View

Preprint (Author's original)This preprint has not been evaluated by subject experts through peer review. Preprints may undergo extensive changes and/or become peer-reviewed journal articles. Open Access

Abstract

MicroRNA (miR)-200c suppresses the initiation and progression of oral squamous cell carcinoma (OSCC), the most prevalent head and neck cancer with high recurrence, metastasis, and mortality rates. However, miR-200c-based gene therapy to inhibit OSCC growth and metastasis has yet to be reported. To develop an miR-based gene therapy to improve the outcomes of OSCC treatment, this study investigates the feasibility of plasmid DNA encoding miR-200c delivered via non-viral CaCO3-based nanoparticles to inhibit OSCC tumor growth. CaCO3-based nanoparticles with various ratios of CaCO3 and protamine sulfate (PS) were utilized to transfect pDNA encoding miR-200c into OSCC cells and the efficiency of these nanoparticles was evaluated. The proliferation, migration, and associated oncogene production, as well as in vivo tumor growth for OSCC cells overexpressing miR-200c were also quantified. It was observed that, while CaCO3-based nanoparticles improve transfection efficiencies of pDNA miR-200c, the ratio of CaCO3 to PS significantly influences the transfection efficiency. Overexpression of miR-200c significantly reduced proliferation, migration, and oncogene expression of OSCC cells, as well as the tumor size of cell line-derived xenografts (CDX) in mice. In addition, a local administration of pDNA miR-200c using CaCO3 delivery significantly enhanced miR-200c transfection and suppressed tumor growth of CDX in mice. These results strongly indicate that the nanocomplexes of CaCO3/pDNA miR-200c may potentially be used to reduce oral cancer recurrence and metastasis and improve clinical outcomes in OSCC treatment. (227 words)

Details

Title: Subtitle: CaCO 3 Nanoparticles Delivering MicroRNA-200c Suppress Oral Squamous Cell Carcinoma
Creators: Qiong J Ding - University of Iowa
Matthew T. Remy - University of Iowa
Chawin Upara - University of Iowa
Jue Hu - University of Iowa
Amanda J. Haes - University of Iowa
Emily Lanzel - University of Iowa
Hongli Sun - University of Iowa
Marisa R. Buchakjian - University of Iowa
Liu Hong - University of Iowa
Resource Type: Preprint
Publication Details: bioRxiv
DOI: 10.1101/2023.10.05.561110
PMID: 37873146
PMCID: PMC10592969
Publisher: Cold Spring Harbor Laboratory
Language: English
Date posted: 10/05/2023
Academic Unit: Roy J. Carver Department of Biomedical Engineering; Oral Pathology, Radiology and Medicine; Prosthodontics; Craniofacial Anomalies Research Center; Oral and Maxillofacial Surgery; Chemistry; Dental Research; Otolaryngology
Record Identifier: 9984500247402771

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