Community dynamics during de novo colonization of the nascent peri-implant sulcus

Purnima Kumar; Tamires Dutra; Nicolas Robitaille; Khaled alTabtbaei; Shareef Dabdoub

doi:10.21203/rs.3.rs-5390824/v1

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Community dynamics during de novo colonization of the nascent peri-implant sulcus

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Community dynamics during de novo colonization of the nascent peri-implant sulcus

Purnima Kumar, Tamires Dutra, Nicolas Robitaille, Khaled alTabtbaei and Shareef Dabdoub

Research square

12/13/2024

DOI: 10.21203/rs.3.rs-5390824/v1

PMCID: PMC11661366

PMID: 39711569

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https://doi.org/10.21203/rs.3.rs-5390824/v1View

Preprint (Author's original)This preprint has not been evaluated by subject experts through peer review. Preprints may undergo extensive changes and/or become peer-reviewed journal articles. Open Access

Abstract

Dental implants have restored chewing function to over 100,000,000 individuals, yet almost 1,000,000 implants fail each year due to peri-implantitis, a disease triggered by peri-implant microbial dysbiosis. Our ability to prevent and treat peri-implantitis is hampered by a paucity of knowledge of how these biomes are acquired and the factors that engender normobiosis. Therefore, we combined a 3-month interventional study of 15 systemically and periodontally healthy adults with whole genome sequencing, fine-scale enumeration and graph theoretics to interrogate colonization dynamics in the pristine peri-implant sulcus. We discovered that colonization trajectories of implants differ substantially from adjoining teeth in acquisition of new members and development of functional synergies. Source-tracking algorithms revealed that this niche is initially seeded by bacteria trapped within the coverscrew chamber during implant placement. These pioneer species stably colonize the microbiome and exert a sustained influence on the ecosystem by serving as anchors of influential hubs and by providing functions that enable cell replication and biofilm maturation. Unlike the periodontal microbiome, recruitment of new members to the peri-implant community occurs on nepotistic principles. Maturation is accompanied by a progressive increase in anaerobiosis, however, the predominant functionalities are oxygen-dependent over the 12-weeks. The peri-implant community is easily perturbed following crown placement, but demonstrates remarkable resilience; returning to pre-perturbation states within three weeks. This study highlights important differences in the development of the periodontal and peri-implant ecosystems, and signposts the importance of placing implants in periodontally healthy individuals or following the successful resolution of periodontal disease.

Details

Title: Subtitle: Community dynamics during de novo colonization of the nascent peri-implant sulcus
Creators: Purnima Kumar - University of Michigan
Tamires Dutra - University of Michigan
Nicolas Robitaille
Khaled alTabtbaei - University of Alberta
Shareef Dabdoub - University of Iowa
Resource Type: Preprint
Publication Details: Research square
DOI: 10.21203/rs.3.rs-5390824/v1
PMID: 39711569
PMCID: PMC11661366
NLM abbreviation: Res Sq
ISSN: 2693-5015
eISSN: 2693-5015
Language: English
Date posted: 12/13/2024
Academic Unit: Dental Research; Periodontics
Record Identifier: 9984759885902771

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