Comparative dynamics of gene expression during in vitro and in vivo Candida albicans filamentation

Rohan S Wakade; Damian J Krysan

doi:10.1101/2023.09.21.558874

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Comparative dynamics of gene expression during in vitro and in vivo Candida albicans filamentation

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Comparative dynamics of gene expression during in vitro and in vivo Candida albicans filamentation

Rohan S Wakade and Damian J Krysan

bioRxiv : the preprint server for biology

Cold Spring Harbor Laboratory

09/21/2023

DOI: 10.1101/2023.09.21.558874

PMCID: PMC10542175

PMID: 37790536

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https://doi.org/10.1101/2023.09.21.558874View

Preprint (Author's original)This preprint has not been evaluated by subject experts through peer review. Preprints may undergo extensive changes and/or become peer-reviewed journal articles. Open Access

Abstract

is one of them most common causes of fungal disease in humans and is a commensal member of the human microbiome. The ability of to cause disease is tightly correlated with its ability to undergo a morphological transition from budding yeast to a filamentous form (hyphae and pseudohyphae). This morphological transition is accompanied by the induction of a set of well characterized hyphae-associated genes and transcriptional regulators. To date, the vast majority of data regarding this process has been based on in vitro studies of filamentation using a range of inducing conditions. Recently, we developed an in vivo imaging approach that allows the direct characterization of morphological transition during mammalian infection. Here, we couple this imaging assay with in vivo expression profiling to characterize the time course of in vivo filamentation and the accompanying changes in gene expression. We also compare in vivo observations to in vitro filamentation using a medium (RPMI 1640 tissue culture medium with 10% bovine calf serum) widely used to mimic host conditions. From these data, we make the following conclusions regarding in vivo and in vitro filamentation. First, the transcriptional programs regulating filamentation are rapidly induced in vitro and in vivo. Second, the tempo of filamentation in vivo is prolonged relative to in vitro filamentation and the period of high expression of genes associated with that process is also prolonged. Third, hyphae are adapting to changing infection environments after filamentation has reached steady-state.

Details

Title: Subtitle: Comparative dynamics of gene expression during in vitro and in vivo Candida albicans filamentation
Creators: Rohan S Wakade - University of Iowa
Damian J Krysan - University of Iowa
Resource Type: Preprint
Publication Details: bioRxiv : the preprint server for biology
DOI: 10.1101/2023.09.21.558874
PMID: 37790536
PMCID: PMC10542175
Publisher: Cold Spring Harbor Laboratory; United States
Grant note: R01 AI133409 / NIAID NIH HHS
Language: English
Date posted: 09/21/2023
Academic Unit: Molecular Physiology and Biophysics; Stead Family Department of Pediatrics; Infectious Disease (Pediatrics)
Record Identifier: 9984557859202771

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