Preprint
Global mapping of the Chlamydia trachomatis conventional secreted effector - host interactome reveals CebN interacts with nucleoporins and Rae1 to impede STAT1 nuclear translocation
bioRxiv : the preprint server for biology
Cold Spring Harbor Laboratory
04/25/2024
DOI: 10.1101/2024.04.25.587017
PMCID: PMC11071493
PMID: 38712050
Abstract
Chlamydia trachomatis ( C.t .), the leading cause of bacterial sexually transmitted infections, employs a type III secretion system (T3SS) to translocate two classes of effectors, inclusion membrane proteins and conventional T3SS (cT3SS) effectors, into the host cell to counter host defense mechanisms. Here we employed three assays to directly evaluate secretion during infection, validating secretion for 23 cT3SS effectors. As bioinformatic analyses have been largely unrevealing, we conducted affinity purification-mass spectrometry to identify host targets and gain insights into the functions of these effectors, identifying high confidence interacting partners for 21 cT3SS effectors. We demonstrate that CebN localizes to the nuclear envelope in infected and bystander cells where it interacts with multiple nucleoporins and Rae1, blocking STAT1 nuclear import following IFN-γ stimulation. By building a cT3SS effector-host interactome, we have identified novel pathways that are targeted during bacterial infection and have begun to address how C.t. effectors combat cell autonomous immunity.
Details
- Title: Subtitle
- Global mapping of the Chlamydia trachomatis conventional secreted effector - host interactome reveals CebN interacts with nucleoporins and Rae1 to impede STAT1 nuclear translocation
- Creators
- Brianna Steiert - University of IowaShelby E Andersen - University of IowaPaige N McCaslin - University of IowaCherilyn A Elwell - University of California, San FranciscoRobert Faris - University of IowaXavier Tijerina - University of IowaParker Smith - University of IowaQuinn Eldridge - University of IowaBrian S Imai - University of Illinois Urbana-ChampaignJustine V Arrington - University of Illinois Urbana-ChampaignPeter M Yau - University of Illinois Urbana-ChampaignKathleen M Mirrashidi - University of California, San FranciscoJeffrey R Johnson - QB3Erik Verschueren - QB3John Von Dollen - QB3Gwendolyn M JangNevan J Krogan - University of California, San FranciscoJoanne N Engel - Universidad Católica de Santa FeMary M Weber - University of Iowa
- Resource Type
- Preprint
- Publication Details
- bioRxiv : the preprint server for biology
- Publisher
- Cold Spring Harbor Laboratory
- DOI
- 10.1101/2024.04.25.587017
- PMID
- 38712050
- PMCID
- PMC11071493
- Language
- English
- Date posted
- 04/25/2024
- Academic Unit
- Molecular Physiology and Biophysics; Microbiology and Immunology
- Record Identifier
- 9984623025202771
Metrics
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