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Improved genotyping of the dysferlin null mouse
Preprint - Protocol   Open access

Improved genotyping of the dysferlin null mouse

Kevin Campbell, Renzhi Han, Kazuhiro Kobuke, Mary Anderson, Daniel Beltrán-Valero de Bernabé, Yvonne Kobayashi and Baoli Yang
Protocol Exchange
Protocol Exchange
04/27/2011
DOI: 10.1038/protex.2011.232
url
https://doi.org/10.1038/protex.2011.232View
Preprint (Author's original)This protocol has been posted on Protocol Exchange, an open repository of community-contributed protocols sponsored by Nature Portfolio. These protocols are posted directly on the Protocol Exchange by authors and are made freely available to the scientific community for use and comment. Open Access

Abstract

The dysferlin-null mouse line that we generated has been an invaluable tool for exploring the function of dysferlin and studying the pathogenesis of dysferlin-deficient muscular dystrophy (1-3). The complete absence of dysferlin protein in this mouse model has been demonstrated by both Western blot and immunofluorescence analyses of skeletal muscle and heart (2, 3), and these mice develop a progressive myopathy that is very similar to dysferlin-deficient muscular dystrophy in patients (3). However, the original published genotyping reaction for dysferlin-null mice can be challenging and requires two separate reactions (3). Here we report an improved genotyping protocol that is reliable and condensed to a single reaction.
 Genotyping dysferlin

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