Mapping the spatial transcriptomic signature of the hippocampus during memory consolidation

Yann Vanrobeys; Utsav Mukherjee; Lucy Langmack; Ethan Bahl; Li-Chun Lin; Jacob J Michaelson; Ted Abel; Snehajyoti Chatterjee

doi:10.1101/2023.01.18.524576

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Mapping the spatial transcriptomic signature of the hippocampus during memory consolidation

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Mapping the spatial transcriptomic signature of the hippocampus during memory consolidation

Yann Vanrobeys, Utsav Mukherjee, Lucy Langmack, Ethan Bahl, Li-Chun Lin, Jacob J Michaelson, Ted Abel and Snehajyoti Chatterjee

bioRxiv : the preprint server for biology

01/19/2023

DOI: 10.1101/2023.01.18.524576

PMCID: PMC9882356

PMID: 36711475

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https://doi.org/10.1101/2023.01.18.524576View

Preprint (Author's original)This preprint has not been evaluated by subject experts through peer review. Preprints may undergo extensive changes and/or become peer-reviewed journal articles. Open Access

Abstract

Memory consolidation involves discrete patterns of transcriptional events in the hippocampus. Despite the emergence of single-cell transcriptomic profiling techniques, defining learning-responsive gene expression across subregions of the hippocampus has remained challenging. Here, we utilized unbiased spatial sequencing to elucidate transcriptome-wide changes in gene expression in the hippocampus following learning, enabling us to define molecular signatures unique to each hippocampal subregion. We find that each subregion of the hippocampus exhibits distinct yet overlapping transcriptomic signatures. Although the CA1 region exhibited increased expression of genes related to transcriptional regulation, the DG showed upregulation of genes associated with protein folding. We demonstrate the functional relevance of subregion-specific gene expression by genetic manipulation of a transcription factor selectively in the CA1 hippocampal subregion, leading to long-term memory deficits. This work demonstrates the power of using spatial molecular approaches to reveal transcriptional events during memory consolidation.

Details

Title: Subtitle: Mapping the spatial transcriptomic signature of the hippocampus during memory consolidation
Creators: Yann Vanrobeys
Utsav Mukherjee
Lucy Langmack
Ethan Bahl
Li-Chun Lin
Jacob J Michaelson
Ted Abel
Snehajyoti Chatterjee
Resource Type: Preprint
Publication Details: bioRxiv : the preprint server for biology
DOI: 10.1101/2023.01.18.524576
PMID: 36711475
PMCID: PMC9882356
Language: English
Date posted: 01/19/2023
Academic Unit: Roy J. Carver Department of Biomedical Engineering; Neurology; Communication Sciences and Disorders; Molecular Physiology and Biophysics; Psychiatry; Psychological and Brain Sciences; Iowa Neuroscience Institute; Neuroscience and Pharmacology; Biochemistry and Molecular Biology
Record Identifier: 9984363657802771

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