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NPS neurons receive extensive input from auditory brainstem nuclei
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NPS neurons receive extensive input from auditory brainstem nuclei

Richie Zhang and Joel C Geerling
bioRxiv
Cold Spring Harbor Laboratory
10/16/2025
DOI: 10.1101/2025.10.08.681223
PMCID: PMC12632321
PMID: 41279263
url
https://doi.org/10.1101/2025.10.08.681223View
Preprint (Author's original) This preprint has not been evaluated by subject experts through peer review. Preprints may undergo extensive changes and/or become peer-reviewed journal articles. Open Access

Abstract

Neurons that produce NPS send output to brain regions implicated in circadian function and threat responses, but less is known about the afferent control of NPS neurons. In this study, we used a conventional retrograde tracer, cholera toxin beta subunit (CTb), to identify afferents to the rostral-lateral parabrachial region that contains the main concentration of NPS neurons. We then used Cre-dependent rabies retrograde tracing in -2A-Cre mice to identify inputs specifically to NPS neurons. -expressing neurons receive heavy input from auditory brainstem structures, including the inferior colliculus, nucleus of the lateral lemniscus, superior olivary complex, and cochlear nucleus. These findings suggest an unexpected role for auditory information in controlling the activity of NPS neurons.

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