Preprint
Polymersomes decorated with SARS-CoV-2 spike protein receptor binding domain elicit robust humoral and cellular immunity
bioRxiv : the preprint server for biology
04/08/2021
DOI: 10.1101/2021.04.08.438884
PMCID: PMC8043456
PMID: 33851166
Abstract
A diverse portfolio of SARS-CoV-2 vaccine candidates is needed to combat the evolving COVID-19 pandemic. Here, we developed a subunit nanovaccine by conjugating SARS-CoV-2 Spike protein receptor binding domain (RBD) to the surface of oxidation-sensitive polymersomes. We evaluated the humoral and cellular responses of mice immunized with these surface-decorated polymersomes (RBD
) compared to RBD-encapsulated polymersomes (RBD
) and unformulated RBD (RBD
), using monophosphoryl lipid A-encapsulated polymersomes (MPLA PS) as an adjuvant. While all three groups produced high titers of RBD-specific IgG, only RBD
elicited a neutralizing antibody response to SARS-CoV-2 comparable to that of human convalescent plasma. Moreover, RBD
was the only group to significantly increase the proportion of RBD-specific germinal center B cells in the vaccination-site draining lymph nodes. Both RBD
and RBD
drove similarly robust CD4
and CD8
T cell responses that produced multiple Th1-type cytokines. We conclude that multivalent surface display of Spike RBD on polymersomes promotes a potent neutralizing antibody response to SARS-CoV-2, while both antigen formulations promote robust T cell immunity.
Details
- Title: Subtitle
- Polymersomes decorated with SARS-CoV-2 spike protein receptor binding domain elicit robust humoral and cellular immunity
- Creators
- Lisa R Volpatti - University of ChicagoRachel P Wallace - University of ChicagoShijie Cao - University of ChicagoMichal M Raczy - University of ChicagoRuyi Wang - University of ChicagoLaura T Gray - University of ChicagoAaron T Alpar - University of ChicagoPriscilla S Briquez - University of ChicagoNikolaos Mitrousis - University of ChicagoTiffany M Marchell - University of ChicagoMaria Stella Sasso - University of ChicagoMindy Nguyen - Pritzker School of Molecular Engineering, University of Chicago, Chicago, IL 60637, United StatesAslan Mansurov - University of ChicagoErica Budina - University of ChicagoAni Solanki - University of ChicagoElyse A Watkins - University of ChicagoMathew R Schnorenberg - University of ChicagoAndrew C Tremain - University of ChicagoJoseph W Reda - University of ChicagoVlad Nicolaescu - University of ChicagoKevin Furlong - University of ChicagoSteve Dvorkin - University of ChicagoShann S Yu - University of ChicagoBalaji Manicassamy - University of IowaJames L LaBelle - University of ChicagoMatthew V Tirrell - Argonne National LaboratoryGlenn Randall - University of ChicagoMarcin Kwissa - University of ChicagoMelody A Swartz - University of ChicagoJeffrey A Hubbell - University of Chicago
- Resource Type
- Preprint
- Publication Details
- bioRxiv : the preprint server for biology
- DOI
- 10.1101/2021.04.08.438884
- PMID
- 33851166
- PMCID
- PMC8043456
- Grant note
- HHSN272201400008C / NIAID NIH HHS T32 GM007281 / NIGMS NIH HHS T32 AI007090 / NIAID NIH HHS F30 CA221250 / NCI NIH HHS T32 HL007605 / NHLBI NIH HHS P30 CA014599 / NCI NIH HHS R01 CA219304 / NCI NIH HHS
- Language
- English
- Date posted
- 04/08/2021
- Academic Unit
- Microbiology and Immunology
- Record Identifier
- 9984297631902771
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