Preprint
The Piezo channel is central to the mechano-sensitive channel complex in the mammalian inner ear
Research Square
American Journal Experts
07/12/2023
DOI: 10.21203/rs.3.rs-2287052/v1
Abstract
The inner ear is the hub where hair cells transduce sound, gravity, and head acceleration stimuli carried by neural codes to the brain. Of all the senses, hearing and balance, which rely on mechanosensation, are the fastest sensory signals transmitted to the central nervous system. The mechanoelectrical transducer (MET) channel in hair cells is the entryway for the sound-balance-brain interface, but the channel's composition has eluded biologists due to its complexity. Here, we report that the mouse utilizes Piezo1 (Pz1) and Piezo2 (Pz2) isoforms as central components of the MET complex. The Pz channel subunits are expressed in hair-cell stereocilia, are co-localized and co-assembled, and are essential components of the MET complex in vitro and in situ, including integration with the transmembrane channel (Tmc1/2) protein. Mice expressing non-functional Pz1 and Pz2, but not functional Pz1 at the ROSA26 locus under the control of hair-cell promoters, have impaired auditory and vestibular traits that can only be explained if Pz channel multimers are integral to the MET complex. We affirm that Pz protein subunits constitute MET channels and that functional interactions with components of the MET complex yield current properties resembling hair-cell MET currents. Our results demonstrate Pz is a MET channel component central to interacting with MET complex proteins. Results account for the MET channel pore and complex.
Details
- Title: Subtitle
- The Piezo channel is central to the mechano-sensitive channel complex in the mammalian inner ear
- Creators
- Jeong-Han Lee - University of Nevada, RenoCristina Perez-Flores - University of Nevada, RenoSeojin Park - University of Nevada, RenoHyo J Kim - University of Nevada, RenoYingying Chen - University of Nevada, RenoMincheol Kang - University of Nevada, RenoJennifer Kersigo - University of IowaJinsil Choi - University of Nevada, RenoPhung Thai - University of California, DavisRyan Woltz - University of California, DavisGuy Perkins - University of California San DiegoChoong-Ryoul Sihn - University of Nevada, RenoBernd FritzschPauline Trinh - University of California, DavisXiao-Dong Zhang - University of California, DavisNipavan Chiamvimonvat - University of California, DavisDolores Perez-Flores - University of Nevada, RenoPadmini Sirish - University of California, DavisYao Dong - University of California, DavisIsaac Pessah - University of California, DavisFeng Wei - University of California, DavisRose Dixon - University of California, DavisBernd Sokolowski - University of South FloridaEbenezer Yamoah - University of Nevada, Reno
- Resource Type
- Preprint
- Publication Details
- Research Square
- DOI
- 10.21203/rs.3.rs-2287052/v1
- Publisher
- American Journal Experts
- Language
- English
- Date posted
- 07/12/2023
- Academic Unit
- Iowa Neuroscience Institute; Biology; Craniofacial Anomalies Research Center
- Record Identifier
- 9984447951902771
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