Abstract
Patterns of Suppressed Mitochondrial Respiration in Isolated Muscle Fibers from Type 2 Diabetics
The FASEB journal, Vol.32(S1), pp.618.26-618.26
04/2018
DOI: 10.1096/fasebj.2018.32.1_supplement.618.26
Abstract
The quality of skeletal muscle is markedly influenced by mitochondrial function. Aberrant mitochondrial function has been associated with reduced contractile and metabolic dysfunction in skeletal muscle, potentially leading to insulin resistance and type 2 diabetes. However, the direct relationship between mitochondrial dysfunction and type 2 diabetes remains to be fully understood. In the present study, vastus lateralis muscle biopsies were obtained from individuals with type 2 diabetes (N = 7) and non‐diabetic, age, gender and weight‐matched controls (N = 7). Maximal mitochondrial oxygen consumption was assessed in isolated muscle fibers and normalized to wet tissue weight. Preliminary findings illustrate that reductions in carbohydratedependent ADP‐stimulated respiration (~30%) were sustained in muscle fibers from type 2 diabetics, even with the addition of the Krebs Cycle anaplerotic substrates, glutamate (~30%) and succinate (~32%). Interestingly, trends of similar magnitudes persisted for lipid substrate metabolism, as fatty acid‐dependent ADP‐stimulated respiration also tended to be lower (~35%) compared to controls. Furthermore, rotenone‐treated respiration in diabetic skeletal muscle tended to be reduced (~37%). To conclude, these initial findings provide support for a systematic impairment in the oxidative metabolism of both carbohydrates and lipids in skeletal muscle of type 2 diabetic individuals. In addition, findings from rotenone‐treated conditions indicate that investigating functional deficits of Complex I of the Electron Transport Chain (ETC) may provide a more insightful understanding of the relationship between mitochondrial respiratory control and type 2 diabetes.
Support or Funding Information
*This study is supported by funds from the American Diabetic Association, ADA‐1‐16‐ICIS‐015, (D.P.C.) and start‐up funds from the Department of Health and Human Physiology at the University of Iowa (V.A.L.).
This is from the Experimental Biology 2018 Meeting. There is no full text article associated with this published in The FASEB Journal.
Details
- Title: Subtitle
- Patterns of Suppressed Mitochondrial Respiration in Isolated Muscle Fibers from Type 2 Diabetics
- Creators
- Kristen D. Turner - Department of Health and Human PhysiologyUniversity of IowaIowa CityIAMatthew P. Harris - University of IowaAna Kronemberger - University of IowaKenichi Ueda - University of IowaAndrew J. Feider - University of IowaHelena Kenny - University of IowaEthan J. Anderson - University of IowaE. Dale Abel - University of Iowa, François M. Abboud Cardiovascular Research CenterDarren P. Casey - University of IowaVitor A. Lira - University of Iowa, François M. Abboud Cardiovascular Research Center
- Resource Type
- Abstract
- Publication Details
- The FASEB journal, Vol.32(S1), pp.618.26-618.26
- Publisher
- The Federation of American Societies for Experimental Biology
- DOI
- 10.1096/fasebj.2018.32.1_supplement.618.26
- ISSN
- 0892-6638
- eISSN
- 1530-6860
- Number of pages
- 1
- Grant note
- Department of Health and Human Physiology at the University of Iowa American Diabetic Association (ADA‐1‐16‐ICIS‐015)
- Language
- English
- Date published
- 04/2018
- Academic Unit
- Fraternal Order of Eagles Diabetes Research Center; Endocrinology and Metabolism; Health and Human Physiology; Dental Research; Physical Therapy and Rehabilitation Science; Internal Medicine; Pharmaceutical Sciences and Experimental Therapeutics; Anesthesia
- Record Identifier
- 9984295034602771
Metrics
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