Dataset
Datasets for Distribution of 2,2’,5,5’-Tetrachlorobiphenyl (PCB52) Metabolites in Adolescent Rats after Acute Nose-Only Inhalation Exposure
University of Iowa
03/22/2024
DOI: 10.25820/data.006680
Abstract
This dataset summarized a study investigating the disposition of PCB52 and its metabolites in male and female rats following nose-only inhalation exposure to PCB52. Briefly, male and female Sprague-Dawley rats (50-58 days of age, 210 ± 27 g; n=6) were exposed for 4 h to PCB52 using a nose-only inhalation exposure system. Sham animals (n=6) were exposed to filtered lab air. Serum was collected immediately after removal of the animals from the nose-only apparatus by cardiac puncture under isoflurane anesthesia. Rats were perfused with saline, and organs were excised, weighed, and stored at -80°C. Based on gas chromatography-tandem mass spectrometry (GC-MS/MS) analysis, animals were exposed to 14 or 23 µg/kg body weight of PCB52. Levels of PCB52 and its hydroxylated metabolites, including 2,2',5,5'-tetrachlorobiphenyl-4-ol (4-OH-PCB52), were measured in tissues using GC-MS/MS. In addition, liquid chromatography-high resolution mass spectrometry (LC-HRMS) analysis was used to identify PCB52 metabolites, including sulfated, methoxylated, and dechlorinated PCB52 metabolites, in tissues.
Details
- Title: Subtitle
- Datasets for Distribution of 2,2’,5,5’-Tetrachlorobiphenyl (PCB52) Metabolites in Adolescent Rats after Acute Nose-Only Inhalation Exposure
- Creators
- Amanda J Bullert - University of IowaXueshu Li - University of Iowa, Occupational and Environmental HealthBinita Gautam - University of Iowa, Occupational and Environmental HealthHui Wang - University of Iowa, Occupational and Environmental HealthAndrea Adamcakova-Dodd - University of Iowa, Occupational and Environmental HealthKai Wang - Institute for Systems BiologyPeter S Thorne - University of Iowa, Civil and Environmental EngineeringHans-Joachim Lehmler - University of Iowa, Occupational and Environmental Health
- Contributors
- Brian Westra (Data Curator) - University of Iowa, Humanities and Social Sciences/Scholarly Impact
- Resource Type
- Dataset
- DOI
- 10.25820/data.006680
- Publisher
- University of Iowa
- Grants
- Language
- English
- Date collected
- 03/17/2021–04/10/2023
- Date published
- 03/22/2024
- Academic Unit
- Civil and Environmental Engineering; Occupational and Environmental Health; Iowa Neuroscience Institute; Humanities and Social Sciences/Scholarly Impact; Iowa Superfund Research Program
- Record Identifier
- 9984448659102771
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