Dataset for Elucidation of the Metabolism of 2,2′,5-Trichlorobiphenyl (PCB18) in Mice Using Fecal Content Screening

Hans-Joachim Lehmler; Sujitha Ravulapalli; Xueshu Li; Youjun Park Suh; Julia Yue Cui

doi:10.25820/data.008258

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Dataset for Elucidation of the Metabolism of 2,2′,5-Trichlorobiphenyl (PCB18) in Mice Using Fecal Content Screening

Hans-Joachim Lehmler, Sujitha Ravulapalli, Xueshu Li, Youjun Park Suh and Julia Yue Cui

University of Iowa

07/07/2026

DOI: 10.25820/data.008258

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Abstract

Lower-chlorinated PCBs (LC-PCBs), such as 2,2′,5-trichlorobiphenyl (PCB18), represent a current environmental health concern; however, their in vivo metabolic fate remains poorly characterized. In this study, 3-month-old male and female mice were orally exposed to PCB18 (6.0 mg/kg body weight) or vehicle (corn oil) daily for three consecutive days. Fecal samples were collected over 0-72 h and analyzed by liquid chromatography–high-resolution mass spectrometry (LC-HRMS)-based fecal content screening to characterize PCB18 metabolite profiles. Monohydroxylated PCB18 (OH-PCB18) was a major metabolite and served as a key intermediate in forming a diverse mixture of dihydroxylated, sulfated, methoxylated, and methoxylated-dihydroxylated metabolites. A dechlorinated hydroxylated PCB18 metabolite was detected as a minor metabolite. Sex-dependent differences were observed in metabolite profiles, consistent with known sex differences in the expression of xenobiotic-metabolizing enzymes. Overall, this work demonstrates the utility of LC-HRMS-based fecal content screening as an effective approach for elucidating the in vivo metabolism of rapidly cleared LC-PCBs.

PCB 18

Polychlorinated Biphenyls

PCB metabolism

Sex-dependent

Xenobiotic-metabolism

Details

Title: Subtitle: Dataset for Elucidation of the Metabolism of 2,2′,5-Trichlorobiphenyl (PCB18) in Mice Using Fecal Content Screening
Creators: Hans-Joachim Lehmler - University of Iowa, Iowa Neuroscience Institute
Sujitha Ravulapalli - University of Iowa, Occupational and Environmental Health
Xueshu Li - University of Iowa, Occupational and Environmental Health
Youjun Park Suh - University of Washington
Julia Yue Cui - University of Washington
Contributors: Brian Westra (Data Curator) - University of Iowa, Humanities and Social Sciences/Scholarly Impact
Resource Type: Dataset
DOI: 10.25820/data.008258
Publisher: University of Iowa
Grants: Airborne PCBs: Sources, Exposures, Toxicities, Remediation, P42ES013661, National Institutes of Health (United States, Bethesda) - NIH
Environmental Health Sciences Research Center, P30ES005605, National Institutes of Health (United States, Bethesda) - NIH
PCB-mediated dysbiosis of the gut microbiome: A missing link in PCB-mediated neurodevelopmental disorders?, R01ES031098, National Institutes of Health (United States, Bethesda) - NIH
Molecular and Cellular Basis of PCB Developmental Neurotoxicity, R01ES014901, National Institutes of Health (United States, Bethesda) - NIH
Developmental flame retardant exposure, gut microbiome, and obesity, R01ES030197, National Institutes of Health (United States, Bethesda) - NIH
Late onset of metabolic liver disease from early life flame retardant exposure and adulthood western diet, F31DK139707, National Institutes of Health (United States, Bethesda) - NIH
Language: English
Date collected: 02/25/2025–02/26/2025
Date published: 07/07/2026
Academic Unit: Public Health Administration; Occupational and Environmental Health; Iowa Neuroscience Institute; Humanities and Social Sciences/Scholarly Impact
Record Identifier: 9985160640902771

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Dataset for Elucidation of the Metabolism of 2,2′,5-Trichlorobiphenyl (PCB18) in Mice Using Fecal Content Screening

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