A pertussis toxin-sensitive GTP-binding protein couples endothelin to phospholipase C in rat mesangial cells

Christie P Thomas; Mark Kester; M J Dunn

doi:10.1152/ajprenal.1991.260.3.F347

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A pertussis toxin-sensitive GTP-binding protein couples endothelin to phospholipase C in rat mesangial cells

Journal article

Peer reviewed

A pertussis toxin-sensitive GTP-binding protein couples endothelin to phospholipase C in rat mesangial cells

Christie P Thomas, Mark Kester and M J Dunn

The American journal of physiology, Vol.260(3 Pt 2), pp.F347-F352

03/1991

DOI: 10.1152/ajprenal.1991.260.3.F347

PMID: 1900389

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Abstract

The mechanisms of stimulation of phospholipase C (PLC) by endothelin, specifically the role of guanine nucleotide-binding proteins (GTP-binding proteins) in coupling the endothelin receptor to PLC, were investigated in rat mesangial cells. Endothelin-1 (ET) synergistically released inositol polyphosphates in the presence of the stimulatory GTP analogue guanosine 5'-O-(3-thiotriphosphate) (GTP gamma S) in permeabilized cells. In addition, in intact cells, pertussis toxin partially inhibited the stimulation of total inositol phosphates (IPn) by ET. Pertussis toxin also reduced the peak ET-stimulated intracellular free calcium level ([Ca2+]i) in these cells, both in the presence and absence of extracellular calcium. Pertussis toxin induced ADP ribosylation of a 41- to 43-kDa protein in mesangial cell membranes, and this effect was inhibited by prior exposure to ET and augmented by the inhibitory GDP analogue, guanosine 5'-O-(2-thiodiphosphate) (GDP beta S). Thus a pertussis toxin-sensitive GTP-binding protein is involved in the activation of PLC by ET in glomerular mesangial cells.

Guanosine 5'-O-(3-Thiotriphosphate) - pharmacology

Endothelins - pharmacology

Inositol Phosphates - antagonists & inhibitors

Calcium - metabolism

Adenosine Diphosphate Ribose - metabolism

Pertussis Toxin

Rats

Type C Phospholipases - metabolism

Inositol Phosphates - pharmacology

Virulence Factors, Bordetella - pharmacology

Endothelins - metabolism

Glomerular Mesangium - cytology

Animals

Glomerular Mesangium - metabolism

GTP-Binding Proteins - metabolism

Details

Title: Subtitle: A pertussis toxin-sensitive GTP-binding protein couples endothelin to phospholipase C in rat mesangial cells
Creators: Christie P Thomas - Department of Medicine, School of Medicine, Case Western Reserve University, Cleveland, Ohio
Mark Kester
M J Dunn
Resource Type: Journal article
Publication Details: The American journal of physiology, Vol.260(3 Pt 2), pp.F347-F352
DOI: 10.1152/ajprenal.1991.260.3.F347
PMID: 1900389
NLM abbreviation: Am J Physiol
ISSN: 0002-9513
eISSN: 2163-5773
Publisher: American Physiological Society; United States
Grant note: HL-22563 / NHLBI NIH HHS
Language: English
Date published: 03/1991
Academic Unit: Stead Family Department of Pediatrics; Obstetrics and Gynecology; Nephrology; Internal Medicine
Record Identifier: 9983986260202771

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