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Analysis of Three-Dimensional Aerosol Deposition in Pharmacologically Relevant Terms: Beyond Black or White ROIs
Journal article   Open access   Peer reviewed

Analysis of Three-Dimensional Aerosol Deposition in Pharmacologically Relevant Terms: Beyond Black or White ROIs

Elliot Eliyahu Greenblatt, Tilo Winkler, Robert Scott Harris, Vanessa Jane Kelly, Mamary Kone and Jose Venegas
Journal of aerosol medicine and pulmonary drug delivery, Vol.28(2), pp.116-129
04/01/2015
DOI: 10.1089/jamp.2013.1120
PMID: 25050754
url
https://www.ncbi.nlm.nih.gov/pmc/articles/4516959View
Open Access

Abstract

Background: This article presents a novel methodological approach to evaluate images of aerosol deposition taken with PET-CT cameras. Traditionally, Black-or-White (BW) Regions of Interest (ROIs) are created to cover Anatomical Regions (ARs) segmented from the high-resolution CT. Such ROIs do not usually consider blurring effects due to limited spatial resolution or breathing motion, and do not consider uncertainty in the AR position within the PET image. The new methodology presented here (Grayscale) addresses these issues, allows estimates of aerosol deposition within ARs, and expresses the deposition in terms of Tissue Dosing (in the lung periphery) and Inner Surface Concentration (in the larger airways). Methods: Imaging data included a PET deposition image acquired during breathing and two CT scans acquired during breath holds at different lung volumes. The lungs were segmented into anatomically consistent ARs to allow unbiased comparisons across subjects and across lobes. The Grayscale method involves defining Voxel Influence Matrices (VIMs) to consider how average activity within each AR influences the measured activity within each voxel. The BW and Grayscale methods were used to analyze aerosol deposition in 14 bronchoconstricted asthmatics. Results: Grayscale resulted in a closer description of the PET image than BW (p<0.0001) and exposed a seven-fold underestimation in measures of specific deposition. The Average Tissue Dosing was 2.11x10(-6) Total Lung Dose/mg. The average Inner Surface Concentration was 45x10(-6) Total Lung Dose/mm(2), with the left lower lobe having a lower ISC than lobes of the right lung (p<0.05). There was a strong lobar heterogeneity in these measures (COV=0.3). Conclusion: The Grayscale approach is an improvement over the BW approach and provides a closer description of the PET image. It can be used to characterize heterogeneous concentrations throughout the lung and may be important in translational research and in the evaluation of aerosol delivery systems.
 Life Sciences & Biomedicine Respiratory System Science & Technology

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