Journal article
Assessment of Polychlorinated Biphenyls and Their Hydroxylated Metabolites in Postmortem Human Brain Samples: Age and Brain Region Differences
Environmental science & technology, Vol.56(13), pp.9515-9526
06/03/2022
DOI: 10.1021/acs.est.2c00581
PMCID: PMC9260965
PMID: 35658127
Appears in UI Libraries Support Open Access
Abstract
Exposure to polychlorinated biphenyls (PCBs) and their hydroxylated metabolites (OH-PCBs) has been implicated in neurodevelopmental disorders. However, the distribution of PCBs and OH-PCBs in the human brain has not been characterized. This study investigated the age-, sex-, and brain region-specific distribution of all 209 PCBs using gaschromatography-tandem mass spectrometry (GC-MS/MS) in neonatal (
= 7) and adult (
= 7) postmortem brain samples. OH-PCB analyses were performed by GC-MS/MS (as methylated derivatives) and, in a subset of samples, by nontarget liquid chromatography high-resolution mass spectrometry (Nt-LCMS). Fourteen higher chlorinated PCB congeners were observed with a detection frequency >50%. Six lower chlorinated PCBs were detected with a detection frequency >10%. Higher chlorinated PCBs were observed with higher levels in samples from adult versus younger donors. PCB congener profiles from adult donors showed more similarities across brain regions and donors than younger donors. We also assess the potential neurotoxicity of the PCB residues in the human brain with neurotoxic equivalency (NEQ) approaches. The median ΣNEQs, calculated for the PCB homologues, were 40-fold higher in older versus younger donors. Importantly, lower chlorinated PCBs made considerable contributions to the neurotoxic potential of PCB residues in some donors. OH-PCBs were identified for the first time in a small number of human brain samples by GC-MS/MS and Nt-LCMS analyses, and all contained four or fewer chlorine.
Details
- Title: Subtitle
- Assessment of Polychlorinated Biphenyls and Their Hydroxylated Metabolites in Postmortem Human Brain Samples: Age and Brain Region Differences
- Creators
- Xueshu Li - University of IowaMarco M Hefti - University of IowaRachel F Marek - Department of Civil and Environmental Engineering, University of Iowa, Iowa City, Iowa 52242, United StatesKeri C Hornbuckle - Department of Civil and Environmental Engineering, University of Iowa, Iowa City, Iowa 52242, United StatesKai Wang - University of IowaHans-Joachim Lehmler - IIHR-Hydroscience and Engineering, University of Iowa, Iowa City, Iowa 52242, United States
- Resource Type
- Journal article
- Publication Details
- Environmental science & technology, Vol.56(13), pp.9515-9526
- DOI
- 10.1021/acs.est.2c00581
- PMID
- 35658127
- PMCID
- PMC9260965
- NLM abbreviation
- Environ Sci Technol
- eISSN
- 1520-5851
- Publisher
- American Chemical Society
- Grant note
- DOI: 10.13039/100000066, name: National Institute of Environmental Health Sciences, award: ES013661, ES027169, ES05605
- Language
- English
- Date published
- 06/03/2022
- Academic Unit
- Civil and Environmental Engineering; Occupational and Environmental Health; Pathology; Iowa Neuroscience Institute; Biostatistics; IIHR--Hydroscience and Engineering; Interdisciplinary Graduate Program in Human Toxicology; Iowa Superfund Research Program
- Record Identifier
- 9984267239802771
Metrics
42 Record Views