Journal article
Development of a Piezoelectric Immunosensor to Rapidly Measure Prohibitin-1 as a Prognostic Biomarker for Sepsis and Other Serious Infectious Diseases
ACS applied electronic materials, Vol.7(24), pp.10955-10963
12/02/2025
DOI: 10.1021/acsaelm.5c01783
PMCID: PMC12746439
PMID: 41473019
Appears in UI Libraries Support Open Access
Abstract
Sepsis is a life-threatening condition and the leading cause of mortality worldwide, particularly when an affected patient develops multiple organ dysfunction syndrome (MODS), a serious complication defined as progressive and potentially irreversible dysfunction in two or more organ systems. Clinical and experimental studies strongly suggest that patient outcomes with sepsis would improve if the risk for MODS and mortality could be identified in the first few hours after the onset of symptoms. We present an approach leveraging quartz crystal microbalance with dissipation monitoring (QCM-D) technology for rapid (similar to 30 min) detection of prohibitin-1 (PHB1), a protein with pleiotropic biological function that dramatically increases in blood during the acute phase of an infection. The feasibility of detection and reproducible quantification of recombinant PHB1 in physiological buffered conditions is described. Sensitivity and detection limit for the third overtone (12.19 Hz mu g-1 mL and 59.06 ng/mL, respectively) were determined to be within the physiological range, highlighting the QCM-D immunosensor's potential for applied use in plasma samples. Importantly, there was strong concordance between PHB1 concentration curves generated using the QCM-D immunosensor and those from enzyme-linked immunosorbent assay (ELISA), which typically requires 36 h. Our findings illustrate a feasible and reproducible method to detect a bloodborne protein using immunosensor technology that has the potential to improve patient outcomes in sepsis and other serious infectious diseases.
Details
- Title: Subtitle
- Development of a Piezoelectric Immunosensor to Rapidly Measure Prohibitin-1 as a Prognostic Biomarker for Sepsis and Other Serious Infectious Diseases
- Creators
- Eman S. Kamel - University of IowaYusra Rahman - University of IowaJolonda C. Mahoney - University of IowaEthan J. Anderson - University of IowaReza Nejadnik - Univ Iowa, Coll Pharm, Dept Pharmaceut Sci & Expt Therapeut, Iowa City, IA 52242 USA
- Resource Type
- Journal article
- Publication Details
- ACS applied electronic materials, Vol.7(24), pp.10955-10963
- DOI
- 10.1021/acsaelm.5c01783
- PMID
- 41473019
- PMCID
- PMC12746439
- NLM abbreviation
- ACS Appl Electron Mater
- ISSN
- 2637-6113
- eISSN
- 2637-6113
- Publisher
- American Chemical Society
- Number of pages
- 9
- Grant note
- R01HL167087 / National Heart, Lung, and Blood Institute; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Heart Lung & Blood Institute (NHLBI) NA / Dr. Ralph and Marian Falk Medical Research Trust
- Language
- English
- Date published
- 12/02/2025
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Pharmaceutical Sciences and Experimental Therapeutics; Fraternal Order of Eagles Diabetes Research Center
- Record Identifier
- 9985090590902771
Metrics
26 Record Views