Journal article
Direct quantification of AD-36 adenovirus DNA by capillary electrophoresis with laser-induced fluorescence
Journal of chromatography. Biomedical applications, Vol.744(1), pp.1-8
07/07/2000
DOI: 10.1016/S0378-4347(00)00219-X
PMID: 10985560
Abstract
An adenovirus, AD-36, has been linked to human adiposity and a sensitive and reliable quantitative method is required to assess AD-36 viral loads. This report describes direct detection of AD-36 viral DNA, which is the first method to quantitate DNA without amplification. Total genomic DNA is hybridized with an AD-36 specific fluorescently labeled probe and analyzed by capillary electrophoresis with laser-induced fluorescence. The minimum detectable quantity is 10.3 ng/ml, corresponding to 282 copies of AD-36 with a precision of 1–6%. These results indicate that direct detection with capillary electrophoresis with laser-induced fluorescence (CE–LIF) is a reliable and sensitive method for quantifying AD-36 viral DNA.
Details
- Title: Subtitle
- Direct quantification of AD-36 adenovirus DNA by capillary electrophoresis with laser-induced fluorescence
- Creators
- Jill M Kolesar - University of Wisconsin–MadisonJudith A Miller - School of Pharmacy, University of Wisconsin, 425 North Charter Street, Madison, WI 53706, USANikhil V Dhurandhar - Departments of Medicine and Nutritional Sciences, University of Wisconsin, 425 North Charter Street, Madison, WI 53706, USARichard L Atkinson - Departments of Medicine and Nutritional Sciences, University of Wisconsin, 425 North Charter Street, Madison, WI 53706, USA
- Resource Type
- Journal article
- Publication Details
- Journal of chromatography. Biomedical applications, Vol.744(1), pp.1-8
- DOI
- 10.1016/S0378-4347(00)00219-X
- PMID
- 10985560
- NLM abbreviation
- J Chromatogr B Biomed Appl
- ISSN
- 0378-4347
- eISSN
- 1872-812X
- Publisher
- Elsevier B.V
- Language
- English
- Date published
- 07/07/2000
- Academic Unit
- Pharmacy; Pharmaceutical Sciences and Experimental Therapeutics
- Record Identifier
- 9984695786902771
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