Journal article
Direct quantification of HIV-1 RNA by capillary electrophoresis with laser-induced fluorescence
Journal of chromatography. B, Biomedical sciences and applications, Vol.697(1-2), pp.189-194
1997
DOI: 10.1016/S0378-4347(97)00334-4
PMID: 9342669
Abstract
HIV-1 RNA was quantitated directly by capillary electrophoresis with laser-induced fluorescence (CE-LIF). CE-LIF was used to analyze cellular RNA and various nucleotide complexes. A fluorescently labeled DNA probe (DNA/RNA complex) in conjunction with thiazole orange intercalator was determined to have optimal stability and sensitivity for RNA analysis. Based on this observation, a hybridization method using a HIV-specific fluorescently labeled probe with analysis by CE-LIF was developed. Plasma samples from a HIV-seropositive patient were lysed to obtain RNA, hybridized with the HIV-specific probe and analyzed by CE-LIF. As little as 19 fg (1710 copies per 1 ml of starting plasma) of HIV RNA can be reliably and quantitatively detected. CE-LIF appears to be an efficient and sensitive method to quantitatively analyze RNA from a variety of sources.
Details
- Title: Subtitle
- Direct quantification of HIV-1 RNA by capillary electrophoresis with laser-induced fluorescence
- Creators
- Jill M Kolesar - School of Pharmacy, University of Wisconsin, 425 North Charter Street, Madison, WI 53706-1515, United StatesPeter G Allen - School of Pharmacy, University of Wisconsin, 425 North Charter Street, Madison, WI 53706-1515, United StatesCynthia M Doran - School of Pharmacy, University of Wisconsin, 425 North Charter Street, Madison, WI 53706-1515, United States
- Resource Type
- Journal article
- Publication Details
- Journal of chromatography. B, Biomedical sciences and applications, Vol.697(1-2), pp.189-194
- Publisher
- Elsevier Science
- DOI
- 10.1016/S0378-4347(97)00334-4
- PMID
- 9342669
- ISSN
- 1387-2273
- Language
- English
- Date published
- 1997
- Academic Unit
- Pharmacy; Pharmaceutical Sciences and Experimental Therapeutics
- Record Identifier
- 9984696551602771
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