Electron paramagnetic resonance study of peripheral blood mononuclear cells from patients with refractory solid tumors treated with Triapine((R))

Jill M. Kolesar; William R. Schelman; Peter G. Geiger; Kyle D. Holen; Anne M. Traynor; Dona B. Alberti; James P. Thomas; Christopher R. Chitambar; George Wilding; William E. Antholine

doi:10.1016/j.jinorgbio.2007.10.013

$Electron paramagnetic resonance study of peripheral blood mononuclear cells from patients with refractory solid tumors treated with Triapine((R))$

Journal article

Peer reviewed

Electron paramagnetic resonance study of peripheral blood mononuclear cells from patients with refractory solid tumors treated with Triapine((R))

Jill M. Kolesar, William R. Schelman, Peter G. Geiger, Kyle D. Holen, Anne M. Traynor, Dona B. Alberti, James P. Thomas, Christopher R. Chitambar, George Wilding and William E. Antholine

Journal of inorganic biochemistry, Vol.102(4), pp.693-698

04/01/2008

DOI: 10.1016/j.jinorgbio.2007.10.013

PMCID: PMC2390869

PMID: 18061679

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Abstract

The metal chelator Triapineo, 3-aminopyridine-2-carboxaldehyde thiosemicarbazone, is a potent inhibitor of ribonucleotide reductase. EPR spectra consistent with signals from Fe-transferrin, heme, and low-spin iron or cupric ion were observed in peripheral blood motionuclear cells (PBMCs) obtained from patients treated with Triapine((R)). One signal that is unequivocally identified is the signal for Fe-transferrin. It is hypothesized that Fe uptake is blocked by reactive oxygen species generated by FeT2 or CuT that damage transferrin or transferrin receptor. A potential source for the increase in the heme signal is cytochrome e released from the mitochondria. These results provide valuable insight into the in vivo mechanism of action of Triapineo. (c) 2007 Elsevier Inc. All rights reserved.

Biochemistry & Molecular Biology

Chemistry

Chemistry, Inorganic & Nuclear

Life Sciences & Biomedicine

Physical Sciences

Science & Technology

Details

Title: Subtitle: Electron paramagnetic resonance study of peripheral blood mononuclear cells from patients with refractory solid tumors treated with Triapine((R))
Creators: Jill M. Kolesar - University of Wisconsin–Madison
William R. Schelman - University of Wisconsin–Madison
Peter G. Geiger - University of Wisconsin–Madison
Kyle D. Holen - University of Wisconsin–Madison
Anne M. Traynor - University of Wisconsin–Madison
Dona B. Alberti - University of Wisconsin–Madison
James P. Thomas - The Ohio State University
Christopher R. Chitambar - Medical College of Wisconsin
George Wilding - Spanish Oncology Genitourinary Group
William E. Antholine - Medical College of Wisconsin
Resource Type: Journal article
Publication Details: Journal of inorganic biochemistry, Vol.102(4), pp.693-698
Publisher: Elsevier
DOI: 10.1016/j.jinorgbio.2007.10.013
PMID: 18061679
PMCID: PMC2390869
ISSN: 0162-0134
eISSN: 1873-3344
Number of pages: 6
Grant note: P41EB001980 / NATIONAL INSTITUTE OF BIOMEDICAL IMAGING AND BIOENGINEERING; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Biomedical Imaging & Bioengineering (NIBIB) EB001980; P41 EB001980-31; P41 EB001980 / NIBIB NIH HHS; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Biomedical Imaging & Bioengineering (NIBIB)
Language: English
Date published: 04/01/2008
Academic Unit: Pharmacy; Pharmaceutical Sciences and Experimental Therapeutics
Record Identifier: 9984695798702771

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